Open Access Original Research Article

Efficacy of Moringa oleifera Leaf Extract for the Treatment of Anemia in Girls

M. B. Shinde, D. R. Waghchoure, S. A. Bhutada, S. B. Dahikar, R. V. Kshirsagar

Journal of Advances in Medical and Pharmaceutical Sciences, Page 1-5
DOI: 10.9734/jamps/2021/v23i530233

The Moringa oleifera has proven to be an ancient medicine for for anemia, skin infections, blackheads, anxiety, and pimples, for intestinal worms, lactation, diabetes and pregnancy. Green leafy vegetables and fruits provide much needed essential micronutrients. Moringa leaves in particular are a rich, inexpensive source of micronutrients. In teenage girls a large number of hormonal changes are witnessed and due to the change in diet which lacks the intake of leafy vegetables and fruits that are essential, a large number of anemic cases are observed. To overcome this problems moringa oleifera leaves extract was formulated. 10 girls of the age group ranging between 17-21 years were selected and their haematological investigation was done. Oral administration of this extract was continued for over a month to these girls. After a month haematological investigation were repeated. Haemoglobin of the moringa extract treated group when compared with the before treatment group were , found out to be increased due to the phytochemical constituents in the extract and also presence of minerals and vitamins.

Open Access Original Research Article

Differential Pressure, P_D, Capital Parameter in the Determination of Cardiac Health and Prominent Kinetic Indicator of Drugs in the Blood

A. B. Kunyima, P. L. Kunyima

Journal of Advances in Medical and Pharmaceutical Sciences, Page 6-14
DOI: 10.9734/jamps/2021/v23i530234

Background: this work is a suite of previous articles where it has been demonstrated that if the differential pressure remains constant, it allows when it is multiplied by cardiac frequency to determine the volumic cardiac power (KUNYIMA equation). Also it has afforded to calculate differential enthalpy (  that is exothermic energy in ejection fraction. In KUNYIMA Formula the differential pressure has allowed to assess in satisfactory way one part of total energy from cellular metabolism (Keith-Flack node) which enable the heart blood to circulate in the organism. In KUNYIMA relations,  made possible the calculation of cardiac exergetic yield nowadays unrecognized by researchers, different from volumic yield defined by ejection fraction. This cardiac exergetic yield has been assimilated to the heart longevity.

Aim and objective: this work gives in detail mathematical useful expressions, rational approaches to be followed when differential pressure substantially changes, for example when the blood contains an injected drug at  initial concentration and when the kinetic of this drug should be followed.

Methodology: Calculations have been our methodology using compartmental analysis.

Results: It is shown hereby the use of differential equations in the determination of kinetic parameters

Conclusion: Physical Cardiochemistry is improved with new theory.

Open Access Original Research Article

Some Heavy Metals Correlated Negatively with Total Antioxidant Capacity, Glutathione Peroxidase, Fructose, and Testosterone in Seminal Plasma of Oligospermic and Azoospermic Males

Edidiong Peter Umoinyang, Ibioku Elekima, Donatus Onwuli

Journal of Advances in Medical and Pharmaceutical Sciences, Page 15-27
DOI: 10.9734/jamps/2021/v23i530235

Aim: The study aimed to investigate the relationship between some heavy metals and total antioxidant capacities, glutathione levels, fructose, and testosterone in seminal plasma of infertile azoospermic and oligospermic males in Akwa Ibom State of Nigeria.

Study Design:  A cross-sectional design with a total of 124 males included in the study of which 32 were azoospermic, 38, oligospermic, and 54, normospermic.

Place and Duration of Study: Semen samples were collected from the urology or fertility clinic of UUTH, St. Luke’s Hospital, Anua, and Ibom specialist hospital of Akwa Ibom State. However, laboratory assays were performed at the Department of Medical Laboratory Science, Rivers State University, Port Harcourt between May, 2018 and January, 2021.

Methodology: Semen specimens were collected after 3-5 days abstinence according to WHO criteria while seminal plasma were obtained from semen by spinning at 4500 rpm for 10 minutes and stored at -700C prior to laboratory analysis. Atomic absorption spectrometer (AAS) was used to determine the levels of heavy metals while ELISA methods were used to determine testosterone and GSH concentrations. TAC and fructose assays were carried out using spectrophotometric methods.

Results: Results showed that non-essential heavy metals such as lead, mercury, and arsenic correlated negatively with testosterone, fructose, and antioxidant activities of the seminal plasma in azoospermic subjects. In addition, lead and mercury correlated positively in the azoospermic subjects. Meanwhile, selenium, an essential heavy metal, correlated positively with testosterone and antioxidant activities in oligospermic subjects with 11-19 x106cells/ml.

Conclusion: The levels of non-essential heavy metals in azoospermic subjects precipitated poor anti-oxidant and testosterone activities inducing oxidative stress while in oligospermic subjects, selenium and antioxidant parameters and testosterone were in good association indicating improved antioxidant activities and testicular function.

Open Access Original Research Article

Synthesis and Pharmacological Evaluation of Novel Benzimidazole Derivatives as Antiulcer and H+ K+ ATPase Inhibitor

Khan Farhan R., Mohd. Saleemuddin Farooqui

Journal of Advances in Medical and Pharmaceutical Sciences, Page 28-39
DOI: 10.9734/jamps/2021/v23i530236

Aims: To Synthesis the novel substituted benzimidazole derivatives and screened Pharmacologically as antiulcer and H+ K+ ATPase inhibitor.

Study Design: Study design as series of substituted benzimidazole derivatives prepared by three different reaction scheme and further evaluated pharmacologically in desired activity.

Place and Duration of Study: In Patal Dhamal Wadwani College of Pharmacy Yavatmal, Maharashtra, India, between July 2014 to August 2017.

Methodology: A series of new 2-[(substituted-pyrimidin-4-yl) sulfinyl]-1H-benzimidazole (54a-54i) were synthesized by the condensation of O-phenylenediamine, KOH and CS2 resulting potassium 1-H-benzimidazole 2-thiolate further treated with glacial acetic acid gives 1H-benzimidazole 2- thiol (2-mercaptobenzimidazole). 4-Chloro 2 Methyl, 6-Alkylpyrimidine- 4-ol prepared by ethanimidamide and alkyl acetoacetate gives 2-Methyl, 6-Alkylpyrimidine-4-ol by chlorination using thionyl chloride 4-Chloro 2 Methyl, 6-Alkylpyrimidine- 4-ol was obtained. further reacted with sodium oxide followed by m-chloroperbenzoic finally gives 2-[(substituted-pyrimidin-4-yl) sulfinyl]-1H-benzimidazole. Structural characterization of these synthesized compounds was confirmed by FT-IR, 1HNMR, and Mass spectral data. Later synthesized derivatives evaluated for their antiulcer and H+ K+ ATPase inhibitory activity by aspirin induced method and assays of H+/K+-ATPase activity respectively.

Results: Compound 54c (74.03%), 54f (72.87%) and 54i (75.15%) shows highly significant antiulcer activity compared to standard drug and compound 54c (88.88 %), 54d (91.03 %), 54f (86.48%) and 54g (84.21%) shows highly significant antisecretory activity when compare to standard drug.

Conclusion: Our research work provokes further to work for development of different derivatives of 2-(pyrimidinyl-sulfinyl) benzimidazole which have lesser side effect and better action than few of marketed drugs.

Open Access Original Research Article

Assessment of Thyroid Hormone Levels in Diabetes Mellitus Subjects Resident in Port Harcourt Metropolis

T. Neebee–Sordum, D. G. Tamuno-emine, D. Onwuli

Journal of Advances in Medical and Pharmaceutical Sciences, Page 40-46
DOI: 10.9734/jamps/2021/v23i530238

Aim: The aim of this study was to assess thyroid hormone levels in among diabetics in Port- Harcourt  of Rivers State in Nigeria

Study Design: This study is a cross-sectional study.

Place and Duration of Study: This study was conducted at Chemical Pathology Department, Rivers State University Teaching Hospital, Port Harcourt, Nigeria, between December, 2019 and February, 2020.

Methodology: A total of 224 subjects were recruited for the study, diabetic parents (132 subjects), non-diabetic parents (112 subjects. Five (5) ml of blood sample was collected and used to analyse for serum thyroxine (T4), tri-iodothyronine (T­­3) and thyroid stimulating hormone (TSH) using Enzyme-linked immunoasorbent assay. Data were analyzed statistically with SPSS version 22.0 and values considered significant at p< 0.05.

Results: The mean ± S.D of serum TSH were 1.28± 0.6u/ml (control) and 1.84±1.05 u/ml (subject), (p=0.023). T4 value were 2.49 ± 0.86u/ml (control) (p=0.024) and 3.90 ±1,62u/ml (subjects) and T3 values were 1.89±0.41u/ml (p=0.0001).

Conclusion: The evaluation of thyroid hormones (TSH, T4 and T3) among diabetes with the age ranges of population studied shows that there was altered thyroid hormone in diabetes mellitus individuals.