Hemodialysis-associated Oxidative Stress: Comparison of Helixone® and Polysulfone® Dialyzer
Mourad Errasfa *
Department of Pharmacology, Faculty of Medicine and Pharmacy, Fez, Morocco and Laboratory of Molecular Basis in Human Pathologies and Therapeutic Tools, University Sidi Mohamed Ben Abdellah, Fez, Morocco
Fatima Zahra Batta
Department of Nephrology and Renal Transplantation, CHU Hassan II, Fez, Morocco
Kaoutar Alaoui Sekkouri
Department of Nephrology and Renal Transplantation, CHU Hassan II, Fez, Morocco
Chakib Maaroufi
Department of Nephrology and Renal Transplantation, CHU Hassan II, Fez, Morocco
Mohamed Arrayhani
Laboratory of Molecular Basis in Human Pathologies and Therapeutic Tools, University Sidi Mohamed Ben Abdellah, Fez, Morocco and Department of Nephrology and Renal Transplantation, CHU Hassan II, Fez, Morocco
Tarik Sqalli Houssaini
Laboratory of Molecular Basis in Human Pathologies and Therapeutic Tools, University Sidi Mohamed Ben Abdellah, Fez, Morocco and Department of Nephrology and Renal Transplantation, CHU Hassan II, Fez, Morocco
*Author to whom correspondence should be addressed.
Abstract
Background: Patients at end stage renal failure require hemodialysis, a process that contributes to oxidative stress, where the quality of hemodialysis membrane plays a key role.
Aim of the Study: We sought to investigate the effect of two different hemodialysis membranes (Helixone® vs Polysulfone®) on several blood biologic and oxidative stress parameters.
Patients and Methods: Among 86 patients of our hemodialysis facility at the University Hospital, 12 patients were included in the study according to inclusion and exclusion criteria. Patients used Polysulfone® membrane in a first step of the study, then they switched to Helixone® membrane. Under the two kinds of membranes, we measured routine biologic parameters, and pre- and post- dialysis plasma oxidative stress parameters (malondialdehyde and Total Anti-Oxidant Activity, as assessed respectively, by the method of thiobarbituric acid, and an anti-oxidant activity commercial kit).
Results: With both Polysulfone® and Helixone® dialyzers, there was an important and significant increase of malondialdehyde upon a single hemodialysis session, however, the increase of malondialdehyde concentration was significantly reduced with Helixone® dialyzer as compared to Polysulfone® one. Plasma total anti-oxidant activity was reduced significantly upon a single hemodialysis session with both dialyzers, however, such a reduction was significantly higher with Polysulfone® (28% reduction) than with Helixone® (11.7% reduction) dialyzer. The levels of other routine blood parameters related to the performance of dialysis process (urea, uric acid, creatinine) under both dialyzers were similar.
Conclusion: Hemodialysis-associated oxidative stress, as assessed by MDA concentration and plasma total anti-oxidant activity, seems to be significantly reduced with Helixone® dialyzer when compared to Polysulfone® dialyzer. This difference may bring positive impact on hemodialysis-associated side effects on cardiovascular and cerebrovascular systems.
Keywords: Hemodialysis, membrane dialyzer, oxidative stress, malondialdehyde