In vitro Antioxidant Activity and Free Radical Scavenging Abilities of Ocimum gratissimum Leaf Extract
Moneme, E. C. *
Department of Biochemistry, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria.
Onochie, A. U.
Department of Biochemistry, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria.
Onuegbu, M. E.
Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
Anyanwu, R. O.
Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
Ajakpofo, F. O.
Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
Oladejo, A. A.
Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Nigeria.
*Author to whom correspondence should be addressed.
Abstract
In recent years, researchers have intensified the search for effective, non-toxic, natural edible plants with free radical scavenging activity whose effects are cushioned by antioxidant molecules. This study evaluated the in vitro antioxidant activity and free radical scavenging abilities of Ocimum gratissimum leaf extract. In vitro antioxidant (nitric oxide, Hydrogen Peroxide, Ferric Reducing property and DPPH) activities were determined using standard methods. Results showed that the leaf extract of Ocimum gratissimum considerably scavenges radicals of nitric oxide, hydrogen peroxide and DPPH in a concentration dependent manner. From the results of the study, it could be concluded that the leaf extract of Ocimum gratissimum could help to ameliorate oxidative stress and consequently improve health.
Keywords: Oxidative stress, free radical, Ocimum gratissimum, health