Synthesis and Determination of Antimicrobial Efficacies of Secondary Metabolite of Streptomyces longisporoflavus and its 3D-Protein Structural Prediction

Olusola Nathaniel Majolagbe *

Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria and Microbiology Programme, College of Agriculture, Engineering and Science, Bowen University Iwo, Osun State, Nigeria.

Felicia Oguntunji

Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria.

Ezekiel Gbadebo Adeyeni

Department of Chemistry, Hallmark University, Ijebu-Itele, Nigeria.

Yetunde Mutiat Feruke-Bello

Department of Microbiology, Hallmark University, Ijebu-Itele, Nigeria.

Louis Odinakose Ezediuno

Department of Microbiology, University of Ilorin, Kwara State, Nigeria.

*Author to whom correspondence should be addressed.


Abstract

Aims: The need for new drug molecules is of high significance considering the rate at which pathogenic bacteria evolve into drug-resistant pathogens. Actinomycetes have been reported as valuable biological agents that possess potent bioactive molecules. This work aims to isolate local strains of actinomycetes in the environment and determine their antimicrobial activities against some clinical isolates.

Study Design: This was an in-vitro study.

Place and Duration of Study: The research was carried out at the Microbial Resources Research Laboratory, Department of Pure and Applied Biology, Ladoke Akintola University of Technology Ogbomoso, Nigeria between January 2020 and December, 2021.

Methodology: Phenotypic and molecular methods of identification of the isolated microorganisms were done. A secondary metabolite of the isolate was obtained. An antibiotic sensitivity test of its metabolites was performed using ten (10) clinical isolates; inhibition zones were measured and recorded for each test organism. Gas chromatography-mass spectrometry (GC-MS) was used to determine the probable bioactive molecules present in the metabolite. The nucleotide sequences of the isolate were translated using Phyre2 and viewed with PyMOL.

Results: The isolate was identified as Streptomyces longisporoflavus. Various zones of inhibition were recorded for each of the tested pathogenic organisms. Five (5) bioactive molecules were identified in the metabolites, with butane-1,1-dibutoxy-2 (1H)-quinolone having the highest peak area. The PYMOL result shows that the protein structure has a 64% identity as a binding molecule.

Conclusion: This study reveals that the local isolates of S. longisporoflavus showed promising antimicrobial potential with bioactive molecules that are potent inhibitors of pathogenic organisms.

Keywords: Streptomyces longisporoflavus, metabolites, antimicrobial potential, bioactive, molecules


How to Cite

Majolagbe, Olusola Nathaniel, Felicia Oguntunji, Ezekiel Gbadebo Adeyeni, Yetunde Mutiat Feruke-Bello, and Louis Odinakose Ezediuno. 2023. “Synthesis and Determination of Antimicrobial Efficacies of Secondary Metabolite of Streptomyces Longisporoflavus and Its 3D-Protein Structural Prediction”. Journal of Advances in Medical and Pharmaceutical Sciences 25 (3):20-30. https://doi.org/10.9734/jamps/2023/v25i3605.

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