Synthesis and Determination of Antimicrobial Efficacies of Secondary Metabolite of Streptomyces longisporoflavus and its 3D-Protein Structural Prediction

Olusola Nathaniel Majolagbe *

Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria and Microbiology Programme, College of Agriculture, Engineering and Science, Bowen University Iwo, Osun State, Nigeria.

Felicia Oguntunji

Department of Pure and Applied Biology, Microbiology Unit, Ladoke Akintola University of Technology, P.M.B. 4000, Ogbomoso, Oyo State, Nigeria.

Ezekiel Gbadebo Adeyeni

Department of Chemistry, Hallmark University, Ijebu-Itele, Nigeria.

Yetunde Mutiat Feruke-Bello

Department of Microbiology, Hallmark University, Ijebu-Itele, Nigeria.

Louis Odinakose Ezediuno

Department of Microbiology, University of Ilorin, Kwara State, Nigeria.

*Author to whom correspondence should be addressed.


Aims: The need for new drug molecules is of high significance considering the rate at which pathogenic bacteria evolve into drug-resistant pathogens. Actinomycetes have been reported as valuable biological agents that possess potent bioactive molecules. This work aims to isolate local strains of actinomycetes in the environment and determine their antimicrobial activities against some clinical isolates.

Study Design: This was an in-vitro study.

Place and Duration of Study: The research was carried out at the Microbial Resources Research Laboratory, Department of Pure and Applied Biology, Ladoke Akintola University of Technology Ogbomoso, Nigeria between January 2020 and December, 2021.

Methodology: Phenotypic and molecular methods of identification of the isolated microorganisms were done. A secondary metabolite of the isolate was obtained. An antibiotic sensitivity test of its metabolites was performed using ten (10) clinical isolates; inhibition zones were measured and recorded for each test organism. Gas chromatography-mass spectrometry (GC-MS) was used to determine the probable bioactive molecules present in the metabolite. The nucleotide sequences of the isolate were translated using Phyre2 and viewed with PyMOL.

Results: The isolate was identified as Streptomyces longisporoflavus. Various zones of inhibition were recorded for each of the tested pathogenic organisms. Five (5) bioactive molecules were identified in the metabolites, with butane-1,1-dibutoxy-2 (1H)-quinolone having the highest peak area. The PYMOL result shows that the protein structure has a 64% identity as a binding molecule.

Conclusion: This study reveals that the local isolates of S. longisporoflavus showed promising antimicrobial potential with bioactive molecules that are potent inhibitors of pathogenic organisms.

Keywords: Streptomyces longisporoflavus, metabolites, antimicrobial potential, bioactive, molecules

How to Cite

Majolagbe, Olusola Nathaniel, Felicia Oguntunji, Ezekiel Gbadebo Adeyeni, Yetunde Mutiat Feruke-Bello, and Louis Odinakose Ezediuno. 2023. “Synthesis and Determination of Antimicrobial Efficacies of Secondary Metabolite of Streptomyces Longisporoflavus and Its 3D-Protein Structural Prediction”. Journal of Advances in Medical and Pharmaceutical Sciences 25 (3):20-30.


Download data is not yet available.


Arifuzzaman M, Khatun MR, Rahman H. Isolation and screening ofactinomycetes from Sundarbans soil for antibacterial activity. African Journal of Biotechnology. 2010:9(29):4615-4619. DOI:10.4236/ns.2013.51002

Das H, et al. Enzymatic screening, antibacterial potential and molecular characterization of Streptomyces isolated from wayanad district in Kerala, India. International Journal of Pharmacyand Bio-Sciences. 2012;2:201-210. Available:

Penka M, Sava T, et al. Characteristics of soil actinomycetes from Antarctica. Journal of Culture Collections. 2002;3:3-14. Available:

Jayaprakashvel M. Therapeutically active biomolecules from marine actinomycetes. J Modern Biotechnol. 2006;1(1):1-7.

Takizawa M, Colwell RT, Hill R. Isolation and diversity of actinomycetes in the Chesapeake Bay. Applied and Environmental Microbiology. 1993;54(4): 997-1002. DOI:10.1128/aem.59.4.997-1002.1993

Ceylan O, Okmen G, Ugar A. Isolation of soil Streptomyces as source of antibiotics active against antibiotics – resistant bacteria, Eur Asia. J. Biosci. 2008;2:72. Available:

Takahashi Y. Exploitation of new microbial resources for bioactive compounds and discovery of new actinomycetes. Actinomycetology. 2004;18:54-61. DOI:10.1128/aem.59.4.997-1002.1993

Nakashima N. et al. Actinomycetes as host cells for the production of recombinant proteins. J. Microb cell; 2005. DOI:10.1186/1475-2859-4-7

Flores FJ, Rinco J, Martin JF. Characterization of the iron regulated desA promoter of Streptomyces pilosus as a system for controlled gene expression in actinomycetes: Effects of trace metal ions on secondary metabolism and the morphological development of Streptomyces. 2003;19:2(1):5 DOI:10.1186/1475-2859-25.

Saadoun I, Hammed KM, Moussauui A. Characterization and analysis of antibiotic activity of some aquatic actinomycetes. Microbros. 1999;93:173-179. DOI: 10.1007/BF02876686

Atta HM, Dabour SM, Desoukey SG. Sparsomycin antibiotic production by Streptomyces sp. AZ-NIOFDI: taxonomy, fermentation, purification and biological activities. American-Eurasian J. Agric & Environ. Sci. 2009;5(3):368-377. DOI:10.9790/3008-1106041922 ISSN 1818-6769

Narayana KJP, Srikanth M, Vijayalakshmi M, Lakshmi N. Toxic spectrum of Aspergillus niger causing black mold rot of onions. Res. J. Microbiol. 2007;2:881-884. DOI:jm.2007.881.884

Kumar N, Singh RK, Mishra SK. Isolation and screening of soil actinomycetes as sources of antibiotics active against bacteria. International Journal of Microbiology Research. 2010;2:12-16. DOI:10.9735/0975-5276.2.2.12-16

Sujatha P, Swethalatha P. Isolation and Screening of novel Streptomyces from sediments of bay of Bengal near Srikakulam coast. Int. J. Curr Pharm Res. 2017;9:40-44. DOI:10.36347/sajb.2022.v10i03.005

Gebreyohannes G, Moges F, Sahile S. Isolation and characterization of potential producing actinomycetes from water and sediments of Lake Tana, Ethiopia. Asian Pac J Trop Biomed. 2013;3(6)426‒435. DOI:10.1016/S2221-1691(13)60092-1

Majolagbe ON, Aina DA, Omomowo IO, Thomas A. Antimicrobial potentials of soil fungi metabolites, molecular dynamics and their 3d- protein structural prediction using bioinformatics tool. International Journal of Current Pharmaceutical Research. 2020a;12(5),20-26. DOI:10.22159/ijcpr.2020v12i5.39759

Majolagbe ON, Aina D.A, Ezediuno L, Ogunmodede O, Kehinde A, Oguntuase B, AdebimpeAdio, Amos Thomas, Kolawole N, Makanjuola E, Adebayo G. Molecular characterization, antimicrobial and larvicidal potentials of fungi isolated from soil samples of Lautech Botanical Garden Ogbomoso, Nigeria. Archive of Science & Technology. 2020b;(1):1-12.

Selvameenal L, Radhakrishnan M, Balagurunathan R. Antibiotic pigment from desert soil actinomycetes biological activity, purification and chemical screening. Indian J. Pharm Sci. 2009; 71:499-504. DOI:10.4103/0250-474X.58174

Rahman MA, Islam MZ, Khondkar P, Islam MA. Characterization and antimicrobial activities of a polypeptide antibiotic isolated from a new strain of Streptomyces parvulus. Bangladesh Pharm. Journal. 2010;13:14-16. Available:

Majolagbe O, Adeniji T, Aina D, Omomowo I, Owoseni A, Adeyeni E, Ogunmodede O. Synthesis of endophytic fungi metabolites, antimicrobial potentials, and detection of their bioactive molecules using gas chromatography-mass spectrometry. Trop J Nat Prod Res. 2022;6(4):572-579.

Gardes M, Bruns TD. ITS primers with enhanced specificity for basidiomycetes- application to the identification of mycorrhizea and rusts. Mol.Ecol. 1993;2: 113-118. Available: 294X.1993.tb00005.x

Vilgalys R, Hester M. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J. Bacteriol. 1990; 172:4238–4246. DOI:10.1128/jb.172.8.4238-4246.

Felsenstein J. PHYLIP – Phylogeny inference package (version 3.2), Cladist. 1989;5:164–166 DOI:10.4236/ajc.2020.82002

Page R. Tree view: An application to display phylogenetic trees on personal computers, Comp. Appl. Biosci. 1996;12: 357–358 DOI:10.1186/1475-2859-4-7.

Kopp J, Schwede T. The swiss-model - respiratory of annotated three-dimensional protein structure homology models. Nucleic acids Res. 2004;2:230-234. DOI:10.1093/nar/gkh008

Lindahl EA. Identification of related proteins on family, superfamily and fold level J Mol Biol. 2000;613-25. DOI:10.1006/jmbi.1999.3377

Hoshino A, Mukai A, Yazawa K. Ranvalencin A. Thiazoidine zinc complex antibiotic produced by a clinical isolate of Norcadiatransverlensis: taxonomy, fermentation, isolation and biological activities. Journal of Antibiotics. 2004;57 (12):797-798. DOI:10.7164/antibiotics.57.797.

Flardh K, Buttner MJ. Streptomyces morphogenesis: dissecting differentiation in a filamentous bacterium. Nat. Rev. microbial. 2009;7(1):36-49. DOI:10.1038/nrmicro1968

Cai Y, Fredenhagen A, Hug P, Meyer T, Peter HH. Further minor metabolites of staurosporine produced by a Streptomyces longisporoflavus strain̋̎. The Journal of Antibiotics.1996;49(6):519-26. DOI:10.7164/antibiotics.49.519

Bridges B, Foster P, Tmms A. Effect of endogenous carotenoid on adaptative mutation in Escherichia coli Fc40̎ Res. 2001;473(1):109-19. DOI:10.1016/s00275107(00)00144-5

Preiss L, Hicks D, Susuki S. Alkaliphilic Bacteria with impact on Industrial Applications, concepts of early life forms and bioenergetics of ATP synthesis. Frontial in Bioengeneering and Biotechnology; 2015. DOI:10.3389/ fbioe.00075

Gurung TD, Sherpa C, Agrawal VP. Isolation and characterization of antibacterial actinomycetes from soil samples of Kalapatthan, Mount Everest Region. Nepal Journal of Science and Technology. 2009;10:173-182.

Cwala Z, Igbinosa EO, Okoh AI. Assessment of antibiotics production potentials in four actinomycetes isolated from aquatic environments of the Eastern Cape province of South African. Afr J. pharm Pharmacol. 2011;5(2):118-124. Available:

Raytapadar S, Paul AK. Production of an antifungal antibiotics by Streptomyces aburauviensis /DA-28, Microbiol Res. 2001;155:315-323. DOI:10.1016/s0944-5013(01)80010-0

Ajijur R, Mohammad ZI, Anwar UL. Antibacterial activities of Streptomyces isolate collected from soils of Rajshahi Bangladesh. Biotechnology research International. 2011;85:7925-79230.