Effective Inhibition of H5N1 Influenza Virus Infectivity using Safe and Calculated Low Power Generated from Life Restoration device under Different Conditions
Waleed Ezzat Madboly *
National Research Center, Center of Scientific Excellence for Influenza Viruses, 12622, Dokki, Giza, Egypt and Zonzia Limited, Research and Development Department, P.O. Box 50777,3609, Limassol, Cyprus.
Ahmed M. Abu-Dief
Department of Chemistry, College of Science, Taibah University, Madinah, P.O. Box 344, Saudi Arabia and Department of Chemistry, Faculty of Science, Sohag University, Sohag-82534, Egypt.
*Author to whom correspondence should be addressed.
Abstract
Objectives: The use of the antiviral drugs and vaccination against pathogenic viruses induce some disorders and death cases. Therefore, it is urgent to develop an alternative therapy to protect and reduce the virus side effects. Here, in this report we designated a novel device termed as Life Restoration Device (LRD) for viral infectivity reduction.
Methodology: Here in this study, we studied the efficacy of the LRD in the treatment of influenza A virus subtype (H5N1) under different conditions using plaque assay analysis.
Results: This report showed that, the infectively inhibition of the H5N1 virus was 90%, 100% and 90% for 30 min, 60 min and 90 min respectively after exposure to LRD equipped with copper rod and cell culture medium. While the copper-coated nickle rod of LRD induces the infectivity inhibition percentage into 95%, 95% and 100% after 30 min, 60 min and 90 min respectively when exposure to LRD and cell culture medium. The nucleic acids analysis using real time PCR for viral solution before and after treatment confirmed the data provided by the plaque assays test. This data confirms the effective inhibition of H5N1 infectivity using specific electric stimulations even under different conditions. Furthermore, the effective treatment of pathogenic viruses such as H5N1 with electric stimulation generated by LRD is effective and safe therapy but needs further future investigations.
Keywords: Life Restoration Device, codified ions, influenza virus, H5N1, viral infectivity inhibition, effective treatment