In vitro Antimitotic Activity and Molecular Docking Studies of Isolated Compounds from Rhynchosia beddomei
M. Lakshmi Madhuri *
Department of Pharmaceutical Chemistry, Gokaraju Rangaraju College of Pharmacy, Bachupally, Hyderabad 500090, Osmania University, Telangana, India and JNTUH, Hyderabad, Telangana, India.
R. Raja Reddy
Department of Pharmaceutical Chemistry, Arya College of Pharmacy, Hyderabad 500090, Osmania University, Telangana, India.
*Author to whom correspondence should be addressed.
Abstract
The goal of this work was to use an exquisite combination of phytopharmacological and modern computational tools to examine the anticancer potential of Rhynchosia beddomei. The methanolic extract of Rhynchosia beddomei was screened for in vitro antimitotic activity like Allium cepa root tip assay. Molecular docking was carried out between the Bcl-2 Receptor, VEGFR-2 and bioactive compounds like apigenein, vitexin, isovitexin, quercetin, vicenin, orientin, rutin etc. Extract of Rhynchosia beddomei showed significant antimitotic activity, by decreasing rate of mitosis in comparison to water. Methotrexate (0.1 mg/mL) was used as a standard and shows highest antimitotic activity. Thus, the selected plant displayed significant antimitotic activity by showing good inhibition. Vitexin, rutin and lucenin have demonstrated remarkable binding affinity towards Bcl-2 and biochanin, isovitexin, orientin and apigenin have demonstrated remarkable binding affinity towards VEGFR-2 respectively. Molecular dynamic simulation studies have further confirmed the finding of docking analysis, suggesting that Bcl-2 and VEGFR-2 can act as an attractive molecular target for vitexin, rutin, biochanin, isovitexin, orientin and apigenin respectively.
Keywords: Rhynchosia beddomei, antimitotic activity, molecular docking, Bcl-2 and VEGFR-2