Methanol Extract of Azanza garckeana Fruit Pulps Protects against Formalin-Induced Reproductive Toxicity in Adult Albino Male Mice
Journal of Advances in Medical and Pharmaceutical Sciences,
Introduction: The aqueous extract of Azanza garckeana was recently reported of exhibiting ameliorative and pro-fertility properties however the protective effects on formalin testicular toxicity have not been studied.
Objective: This study investigated the protective effect of methanol extract of Azanza garckeana on formalin-induced testicular toxicity.
Methods: Forty male albino mice were randomly divided into 8 groups of 5. Animals in the first group (1) served as control and administered normal saline (1 ml/kg) by the oral route daily for 40 days. In similar manner, animal in groups 2 received formalin (10 mg/kg) by the IP route, while animals in groups 3; 4 and 5 concurrently received formalin (10 mg/kg IP) and extract at doses of 125; 250 and 500 mg/kg respectively by the oral route. Mice in groups 6; 7 and 8 received the extract at doses of 125; 250 and 500 mg/kg respectively. Phytochemical analysis was conducted for each constituent using specific methods. Gonadotropin and sperm analysis were carried out using standard methods.
Result: Phytochemical screening revealed the presence of various constituents, but notably flavonoids. Induced-toxicity with formalin and concurrent treatment with extract at doses of 250 and 500 mg/kg from day 20 to 40 caused significant body weight increase compared to baseline (p < 0.05). Similarly, treatment with the extract alone at all doses caused significant increase in body weight from day 20 to 40 (p < 0.05). Treatment with the extract at 250 and 500 mg/kg, caused a significant increase in weight of testes and epididymis compared to control and untreated group (p < 0.05).The extract induced significant increase in gonadotropin levels of animals compared to control and the untreated group (p < 0.05).The extract at 125 mg/kg demonstrated the highest fecundity potential, but there was no any consistent relationship between GSI and fecundity.
Conclusion: This investigation was able to establish the protective and pro-fertility potentials of methanol extract of Azanza garckeana.
- Azanza garckeana
- reproductive toxicity
- gonadosomatic index
How to Cite
IARC. Formaldehyde, 2-butoxyethanol and 1-tertbutoxypropan-2-ol. International Agency Research on Cancer monograph on the evaluation of carcinogenic risk to humans. 2006;88.
Duong A, Steinmaus C, McHale CM, Vaughan CP, Zhang L. Reproductive and developmental toxicity of formaldehyde: a systematic review. Muta Res. 2011;728(3):118-138.
Zhou D, Zhang J, Wang HX. Assessment of potential reproductive toxicity of long-term exposure of adult male rats to low-dose formaldehyde. Toxicol Industr Health. 2011;27(7):591-598.
Wang HX, Li HC, Lu MQ, Zhou DX, Bai LZ, Du LZ, Xue X, Lin P, Qiu SD. Association between occupation exposure to formalin and semen quality. Sci Rep 2015;5:15874.
Vosoughi S, Khavanin A, Saleman M, et al. Adverse effect of formaldehyde vapor on mouse sperm parameters and testicular tissues. Int J Fertil Steril. 2013;6(4):250-267.
Razi M, Maleknejad H, Sayrafi R, Hosseinchi MR, Feyzi S, Moashtagion SM, Janbaz H. Adverse effects of long-term exposure to formaldehyde vapor on testicular tissues and sperm parameters in rats. Vet Res Forum. 2013;4(4):213-219.
Kose E, Sarsilmaz M, Tas U, Kavakli A, Turk G, Ozlem D, Sapmaz H, Ogeturk M. Rose oil inhalation protects against formaldehyde-induced testicular damage in rats. Andrologia. 2011;44(S1):342- 348.
Zhou, DX, Qiu SD, Zhang J, Wang ZY. Reproductive toxicity of formaldehyde to adult male rats and the functional mechanisms concerned. Sinchuan Da-Xue Xue-Bao Yi-Xue-Bao. 2006;27(4): 566-569.
Kumar N, Sigh AK. Trends of male factor infertility, an important cause of infertility: a review of literature. J Hum Reprod Sci. 2015;8(4).191-196.
Alkhedaide A, Alshehri ZS, Sabri A, Abdel-Gafar T, Soliman MM, Attia H. Protective effect of grape seed extract against cadmium-induced testicular dysfunction. Mol Med Rep. 2016;13:3101-3109.
Irvin DS. Epidemiology and etiology of male infertility. Human Reprod. 1998;13(Suppl 1):33-44.
Swan SH, Elkin EP, Fenster L. The question of declining sperm density revisited: an analysis of 101 studies published 1934-1996. Environ Health Perspect, 2000;108, 961-966.
Brake A, Krause W. Decreasing quality of semen. Br Med J. 1992;305:1498.
Bukar BB, Tsokwa NE, Orshi ODG. Ameliorative and fecundity potentials of aqueous extract of Azanza garackeana fruit pulp in formalin-induced toxicity on male albino mice. J Pharm Biores. 2020:17(2):164-173.
National Institute of Health (NIH). Guide for care and use of laboratory animals, 8th Edition, 2011, The National Academic Press, Washington DC, USA.
Adegboye MF, Akinpelu DA, Okoh A. The bioactive and phytochemical properties of Garcinia kola seed extract on some pathogens. Afr J Biotech. 2008; 7(21):3934-3938.
Trease G, Evans WC. Pharmacognosy, Baillere Tindall, London, 12th Edition, 1983, pp 387, 475-476
Otsuka H. Purification by solvent extraction using partition coefficient. In: SD Sarker, Z Lafif and GI Alexander (Eds). Methods in Biotechnology: Natural Product Isolation, 2nd Ed, 2006, Human Press Inc, Totowa, New Jersey, USA.
Wall ME, Eddy CR, McClemann ML. Detection and estimation of steroid-saponin in plant tissues. Liebigs Annalen de Chemie. 1952;23:1337-1338.
Sofowora A. Medicinal plants and traditional medicine in Africa. John Wiley and Sons, New York, in association with Spectrum Books Ltd, Ibadan, Nigeria 1982;142-145.
Odebedy O, Sofowora A. Phytochemical screening of Nigerian medicinal plants. Lloydia. 1978;41:41-234.
Braide VP, Agube GE, Udoh FV. Effect of Garcinia kola seed alkaloid extract on levels of gonadal and pituitary gonadotropins in rat serum. Nig J Physio Sci. 2003;18(1-2):59-64.
Gan, S. D., and Patel, K. R.(2013). Enzyme immunoassay (EIA) and enzyme-linked immunosorbent assay (ELISA). J Invest. Dermatol. 2013;133: e12.
Bavister B, Andrew, JC. A rapid sperm motility bioassay procedure for quality control testing of water and culture media. J In-vitro Fertil Embr Transfer. 1998;5:67-75.
Nantia EA, Moundipa PF, Monsees TK, Carreau S. Medicinal plants as potential male anti-fertility agents: A review. Basic Clin Androl. 2009;19:148-158.
Adewoyin A, Ibrahin M, Roszaman R, Isa ML, Alewi NA, Rafa AA, Anuar MN. Male infertility: the effect of natural antioxidants and phytocompounds on seminal oxidative stress. Diseasea. 2017;5:9.
Abarikwu SO, Mgbudom-Okah CJ, and Onuah CL. The protective effect of rutin against Busulfan-induced testicular damage in adult rat. Drug Chem Toxicol; 2020.
Torres-Arce ET, Vizmanos B, Babio N, Marquez-Sandoval F, Salas-Huctos A. Dietary antioxidants in the treatment of male infertility: counteracting oxidative stress. Biol 2021;10:241.
Jamalan M, Ghafane MA, Hoeinzadeh P, Hashemitabar M, Zeinali M. Human sperm quality and metal toxicants: protective effects of some flavonoids on male reproductive function. Int J Fertil Steril. 2015;9(3):215-222.
Ye RJ, Yang JM, Hai DM, Liu N, Yu JQ. Interplay between male reproductive dysfunction and the therapeutic effect of flavonoids. Fitoterapia. 2020;147:104756.
Palmer NO, Bakos HW, Fullston T, Lame M. Impact of obesity on male fertility, sperm function and molecular composition. Sperm. 2012;2(4):253-263.
Katib A. Mechanisms linking obesity to male infertility. Centr Euro J Urolo. 2015: 68:79-85.
Ehala-Aleksejev K, Punab M. Relationship between total testicular volume, reproductive parameters and surrogate measures of adiposity in men presenting for couple’s infertility. Andrologia. 2017;50(4):e12952.
Elbashir S, Magdi Y, Rashed A, Henkel K, Agarwal A. Epididymal contribution to male infertility: an overlooked problem. Andrologia. 2020;e13721.
Amann RP, Hammersted, RH, Veeramachaneni DN. The epididymis and sperm maturity: a perspective. Reprod Fertil Dev. 1993;5(4):361-381.
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