L-Citrulline Supplementation Enhances Reproductive Functions of Lead Acetate Induced Testicular Toxicity in Male Sprague Dawley Rats

Main Article Content

Tolunigba Abisola Kolawole
Beatrice Olatundun Oluwatayo
Ogadinma Ilochi
Elizabeth Umoren
Datonye Victor Dapper

Abstract

Introduction: Lead (Pb) is a transition metal and a known male reproductive toxicant that induces its effects mainly through oxidative stress. This study investigated the effects of L-Citrulline (Cit) supplement on reproductive functions and antioxidant activities in Lead acetate treated male rats. Materials and Methods: Twenty  male rats (180-200 g) were grouped into four  and treated as follows; Group 1 (Control), given distilled water, Group 2 was given Pb acetate (2.25mg/kg), Group 3 was co-treated with Pb acetate (2.25mg/kg) and Cit (900mg/kg) and Group 4 was given Cit (900mg/kg) only. All administrations were done orally for thirty days. Caudal sperm, serum hormone levels and testicular antioxidant activities and Nitric oxide levels were evaluated at the end administrations.

Results: Results showed decrease (p<0.05) in sperm morphology, count, viability, motility, FSH, LH, Testosterone, Catalase, Nitric Oxide and Super oxide dismutase in Group 2(Pb treated rats), when compared to Group 1(Control).However, in Group 3 (Pb acetate co-administered with Cit) the effect was significantly reversed (p<0.05) compared with Group 2 and significant increase was observed in Group 4 (Cit only group) compared with Group 2.

There was an increase (p<0.05) in Malondialdehyde level in Group 2 compared with (Group 1) Control while a significant decrease (p<0.05) was observed in Groups 3 and 4 compared with Group 2.

Conclusion: The results of this study suggest that L- Citrulline supplement has ameliorating capacity on the toxic effect of Lead acetate on sperm parameters.

Keywords:
L- Citrulline, lead acetate, sperm parameters, reproductive hormones, antioxidants, rats.

Article Details

How to Cite
Kolawole, T. A., Oluwatayo, B. O., Ilochi, O., Umoren, E., & Dapper, D. V. (2020). L-Citrulline Supplementation Enhances Reproductive Functions of Lead Acetate Induced Testicular Toxicity in Male Sprague Dawley Rats. Journal of Advances in Medical and Pharmaceutical Sciences, 22(12), 22-35. https://doi.org/10.9734/jamps/2020/v22i1230208
Section
Original Research Article

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