Anthill Inhibiting Bacteria, a Promising Source of Bio Efficacy

Main Article Content

A. A. Katun
A. R. Abdulmumin
M. U. Yahaya
N. K. Habeeb
A. Bala

Abstract

The investigation into soil bacteria has been widely studied and becoming increasingly appreciated as an exceptional reservoir of unique naturally occurring biologically active metabolites with pharmaceutical applications. This article aimed to isolate, identify and biochemically characterize antibiotic-producing bacteria from anthill soils in the permanent site of Ibrahim Badamasi Babangida University, Lapai (IBBUL), Niger State, Nigeria. The sum of ten samples were collected from five sampling sites, the sampling was done in threefold (morning, noon and evening) and analyzed adopting standard microbiological protocols. The obtained result revealed that the total bacteria count in the morning ranges from 2.1×107 cfu/mL to 1.4×106 cfu/mL, noon count ranges from 3.1×107 to 2.6×106 cfu/mL while evening count was in the range of 2.1×107 cfu/mL to 1.7×106 cfu/mL. A total number of five (5) bacteria were isolated as Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis, Bacillus lentus and Micrococcus reseus. The total prevalence of the bacterial isolates in the morning, noon and evening were calculated as B. subtilis (109.08%), S. epidermidis (36.36%), M. reseus (36.36%), B. lentus (63.63%), and S. aureus (54.54%) respectively. These isolates were further assayed against Escherichia coli, Salmonella typhi, Klebsiella sp. and Staphylococcus aureus. The antibacterial outcome showed that two (2) (40%) anthill isolates exhibited antibacterial activity against three (3) tested bacteria (Escherichia coli, Salmonella typhi and Staphylococcus aureus). This research study has showcased that the production of inhibitory substances are common among some of the bacterial strains isolated from anthills.

Keywords:
Bio efficacy, antibiotic-producing bacteria, anthill and inhibition.

Article Details

How to Cite
Katun, A. A., Abdulmumin, A. R., Yahaya, M. U., Habeeb, N. K., & Bala, A. (2020). Anthill Inhibiting Bacteria, a Promising Source of Bio Efficacy. Journal of Advances in Medical and Pharmaceutical Sciences, 22(12), 14-21. https://doi.org/10.9734/jamps/2020/v22i1230207
Section
Original Research Article

References

Bala JP, Abioye HS, Auta D, Damisa FA, Kuta NU, Adabara G, Udenyi EO. Isolation and screening of soil Bacteria with the potential to produce antibiotics. Journal of Biological Science. 2012;6(2):0974–7532.

Samuel L, Chai SL, Ismail A, Fazia MS, Kok H and Awang AS. Antimicrobial producing microbes isolated from soil samples collected from Nanga merit forest in Sarawak, Malaysian borneo. European Journal of Experimental Biology. 2014; 4(1):494-501.

Uwalaka EI, Kassim ZJ, Abdulmumin AR and Hamzat A. Isolation and identification of antibiotic producing bacteria from soil samples of abattoir in lapai, niger state, nigeria. Journal of Applied Life Sciences International. 2019;22(2):1-8.

Nejad EMA, Abtahi A, Zareian G. European Journal of Experimental Biology.2013;3(5):213-217.

Dheerendra K, Ankita S, Niharika C, Amit P, Sunil K. Isolation, screening and characterization of bacteria having antibacterial activity from industrial waste effluent american. Journal of Biomedical Sciences 2018;10(1):9-17.

Demain AL. Small bugs, big business: the economic power of the microbe. Biotechnolgy Advancement. 2000;18(6): 499-514.

Drablos F, Nicholson DG, Ronning ME. Study of zinc coordination in Bacitracin A. Biochim. Biophys. Acta. 1999;14(1):433-442.

Raji AA, Jummai KZ, Busu MS, Yakubu Y, Ibrahim K. In-vitro antimicrobial susceptibility and phytochemical constituents of methanol leaf extract of Prosopis africana against some selected microorganisms. Journal of Advances in Microbiology. 2019;18(1):1-8.

National Population Commission (NPC). Nigeria Demographic and Health Survey 2006 Abuja, Nigeria, Report; 2008.

Kaur S., Kaur J, Pankaj PP. Isolation and characterization of antibiotic producing

microorganisms from soil samples of a certain area of Punjab region of India. International Journal of Pharmaceutical and Clinical Research.2014;6(4):312-521.

Kavitha R, Dh Amodharan N, Dhivya C. Screening, isolation and antibacterial activity of antibiotic producing Bacteria obtained from saprophytic soil samples. Asian Journal of Pharmaceutical and Clinical Research. 2017;10(3):92-96.

Mehmet ST, Yusuf Y, Baris O, Nilay G. Bacteria found on banks automated teller machines (ATMs). African Journal of Microbial Research. 2013;7:1619-1621.

Atlas RM. Principles of Microbiology, 2nd edition. WmC. Brown Publishers, Dubuque; 1997.

Bergeys RJ. Bergey's Manual of Systematic Bacteriology (second edition) volume 5 and the study of actinomycetes systematic in China. 2013;53(6):521-530.

Marshall TR and Devinny JS. The Microbial ecosystem in petroleum waste land treatment. Jounal of water science technology. 2008;20(11):285-291.

Shang-Ping K and Harry KK. Effect of soil temperature, moisture, and relative humidity on entomopathogenic nematode persistence. Journal of Invertebrate Pathology. 1991;57(2):242-249.

Kotova AA, Golichenkov TG and Dobrvol’skaya H. Microbiological activity in the anthills of fallow lands (Ryazan Region). Moscow University Soil Science Bulletin. 2013;68:78-81.

Rafiq A, Khan SA, Akbar A, Shafi M, Ali I, Rehman FU, Rashid R, Shakoor G, Anwar M. Isolation and identification of antibiotic producing microorganisms from soil. International Journal of Pharmaceutical Sciences and Research. 2018;9(3):1002-11.

Tatiana AE, Alla AG, Mariia VD, Yuliya VB, Natalia DM, Irina GS, Byazilya FV, Eugene AR, Igor AI, Vladislav AK, Olga VE. Antimicrobial activity of microoganisms isolated from ant nests of Lasius niger. MDPI, Life. 2020;4(10):91.

Acebal C, Alcazar R, Canedo M, Rodringuez P, Romero F, Frenandez JL. Two marine agrobacterium producers of sesbanimide antibiotics. J. Antibiot. 2007;51(1):64-67.

Sumi CD, Yang BW, Yeo IC and Hahm YT. Antimicrobial peptides of the genus Bacillus: a new era for antibiotics. Can. J. Microbiol. 2015;61:93–103.

Sura IAJ, Nagham AGJ, Raad AA, Raflaa SHH. Isolation and identification of antibiotic producing bacteria from local soil in Babylon province. Euphrates Journal of Agriculture Science. Second veterinary Conference. 2017;304-310.

Kevin BK and Cody AP. Characterization of antibiotic-producing bacterium isolated from anthill sediment with activity against eskape pathogens. Fine Focus. 2018;4(2): 189-201.

Ini DE, Uduak CU, Samuel AI. Changes in soil thermal properties and microbial load of ultisols under cassava mill effluent discharge in Uyo, Nigeria. EC Agriculture. 2020;68:03-10.

Sharga BM, Nikolaychuk VI, Maga MI. Comparative IR Spectrometry of antimicrobial substances derived antibiotic from Bacillus. Mikpobiojiotir. 2004;15:75-77.

Moshafi MH, Forootanfar H, Ameri A, Shakibaie M, Dehghan-Noudeh G, and Razavi M. Antimicrobial activity of Bacillus sp strain FAS1 isolated from soil. Pakistan J. Pharm. Sci. 2011;24:269–275.

Amin M, Rakhisi Z and Ahmady AZ: Isolation and identification of Bacillus species from soil and evaluation of their antibacterial properties. Avicenna Journal of Clinical Microbiology and Infection 2015;2(1):18-25.

Akbar A, Ali I and Anal AK: Industrial perspectives of lactic acid bacteria for biopreservation and food safety. The Journal of Animal and Plant Sciences, 2016;26(4):938-948.

Nasfi Z, Busch H, Kehraus S, Linares-Otoya L, König GM, Schäberle TF and Bachoual R. Soil Bacteria Isolated From Tunisian Arid Areas Show Promising Antimicrobial Activities Against Gram-Negatives. Front. Microbiol. 2018;9:27-42.

Ahmed RN, Sani AH, Ajijolakewu M. and Alamu FB. Soil screening for antibiotic – producing microorganisms. Adv Environ Biol. 2013;7(1):7-11.

Samuel L, Chai Sin L, Ismail A., Fazia MS, Ng KH and Awang AS. Antimicrobial producing microbes isolated from soil samples collected from Nanga merit forest in sarawak, Malaysian Borneo. European Journal of Experimental Biology. 2014;4(1):494-501.

Anita M, Hari SL and Balawant SR. Isolation of antimicrobial producing bacteria from soil samples collected from Bhopal Region of Madhya Pradesh, India. International .Journal of .Current Microbiology and Applied Sciences. 2014; 3(12):563-569.

Sonenshein AL, Hoch JA and Losick R: Bacillus subtilis and its closest relatives. From genes to cells. American Society for Microbiology. 2002;3-5.

Prescott ML, Harley PJ and Klein AD: Microbiology. 7th ed. Publishing Group 2008.

Baruzzi F, Quintieri L, Morea M and Caputo L. Antimicrobial compounds produced by Bacillus sp and applications in food. Science Against Microbial Pathogens: Communicating Current Research and Technological Advances 2011;2:1102-11.