The Therapeutic Potential of Time-Restricted Fasting on Experimental Ulcerative Colitis

Main Article Content

Serah Funke Ige
Idowu Adebola Okanlawon
Oluwatomiwa Toby Adio
Oluwatosin Funmi Ojoye

Abstract

Aim: Intermittent fasting have been reported to have beneficial effect, in that it improves gut microbiota and lowers inflammation. This research is, however, targeted at evaluating the healing effects of fasting on ulcerative colitis in rats.

Study Design: A total of eighteen Wistar rats were used for this study, and were divided into three major groups; animals that were neither induced with colitis nor fasted (group 1), animals with colitis and were allowed to fast (group 2), animals with colitis but were not allowed to fast (group 3).

Place and duration of study: Department of Physiology, Ladoke Akintola University of Technology, Ogbomoso, Oyo State, between March, 2020 and July, 2020

Methodology: The weight of the 18 animals used was 180 ± 20 g. Colitis was induced by a single dose of intra-rectal administration of 1mL/100g body weight of 6% acetic acid. Animals in group 1 served as control animals. Animals in group 2 were only given access to food between 4:00 p.m and 6:30 p.m, whereas animals in group 3 were given food and water ad libitum. Animals were sacrificed ten days post colitis induction. Colonic levels of Tumor necrotic factor-alpha (TNFα), Glutathione (GSH) concentration, Superoxide dismutase (SOD), Catalase (CAT) and Myeloperoxidase (MPO) activities were measured. The blood glucose level in the animals was also recorded by the use of a glucometer.

Results: There was a reduction in the concentration of TNFα and GSH, an increase in CAT and SOD activities in the colitis animals that were allowed to fast when compared with colitis animals that were not allowed to fast, ten days post colitis induction. There was also the lowering of blood sugar level, all signifying the beneficial effect of fasting on chemically-induced colitis. Thus, intermittent fasting helped the animals to heal from chemically-induced colitis.

Keywords:
Ulcerative colitis, intermittent fasting, inflammation, antioxidants.

Article Details

How to Cite
Ige, S. F., Okanlawon, I. A., Adio, O. T., & Ojoye, O. F. (2020). The Therapeutic Potential of Time-Restricted Fasting on Experimental Ulcerative Colitis. Journal of Advances in Medical and Pharmaceutical Sciences, 22(8), 25-33. https://doi.org/10.9734/jamps/2020/v22i830188
Section
Original Research Article

References

Roy U, Gálvez EJ, Iljazovic A, Lesker TR, Błażejewski AJ , Pils MC, HeiseU, Huber S, Flavell RA, Strowig T. Distinct microbial communities trigger colitisdevelopment upon intestinal barrier damage via innate or adaptive immune cells. Cell Rep. 2017;21:994–1008.

El-TawilAM. Epidemiology and inflammatory bowel diseases. World J Gastroenterol. 2013;19(10):1505–1507. DOI: 10.3748/wjg.v19.i10.1505

Adams SM, Bornemann PH. Ulcerative colitis. Am Fam Physician. 2013;87:699–705.

Crawford PA, Crowley JR, Sambandam N, Muegge BD, Costello EK, Hamady M, Knight R, Gordon JI. Regulation of myocardial ketone body metabolism by the gut microbiota during nutrient deprivation. Proceedings of the National Academy of Sciences. 2009;106(27):11276-81. Available:https://doi.org/10.1073/pnas.0902366106

Tinsley GM, La Bounty PM. Effects of intermittent fasting on body composition and clinical health markers in humans. Nutr Rev. 2015 73:661–674.

Patterson RE, Laughlin GA, LaCroix AZ, Hartman SJ, Natarajan L, Senger CM, Martínez ME, Villaseñor A, Sears DD, Marinac CR. Intermittent fasting and human metabolic health. J AcadNutr Diet. 2015;115:1203–1212.

Choi Choi I, Changhan L, Longo V. Nutrition and fasting mimicking diets in the prevention and treatment of autoimmune diseases and immunosenescence.Mol and Cell.Endocrinol. 2017;455:4-12.

Chen CW, Villani V, Buono R, Wel M, Kumar S, Yilmaz OH, Cohen P, Sneddon JB, Perin L, Longo VD. Fasting-mimicking diet promotes Ggn3-driven β-cell regeneration to reverse diabetes. Cell. 2017;168:775-788e12.

Li G, Xie C, Lu S, Nichols RG,Tian Y, Li L , Patel D, Ma Y, Brocker CN, Yan T, Krausz KW, Xiang R, Gavrilova O, Patterson AD, Gonzalez FJ. Intermittent fasting promotes white adipose browning and decreases obesity by shaping the gut microbiota. Cell Metabol. 2017;26:672–685 e674.

Patterson RE, Sears DD. Metabolic effects of intermittent fasting. Annu Rev Nutr. 2017;37:371–393.

Wei S, Han R, Zhao J, Wang S, Huang M, Wang Y, Chen Y. Intermittent administration of a fasting-mimicking diet intervenes in diabetes progression, restores beta cells and reconstructs gut microbiota in mice. NutrMetab. 2018;15:80.

Brandhorst S, Choi IY, Wei M, Cheng CW, Sedrakyan S, Navarrete G, Dubeau L, Yap LP, Park R, Vinciguerra M, et al. A Periodic Diet that Mimics Fasting Promotes Multi-System Regeneration, Enhanced Cognitive Performance, and Healthspan. Cell Metab. 2015;22(1):86-99.

Wei M, Brandhorst S, Shelehchi M, Mirzaei H, Cheng CW, Budniak J, Groshen S, Mack WJ, Guen E, Di Biase S, et al. Fasting mimicking diet and markers/risk factors for aging, diabetes, cancer, and cardiovascular disease. Sci. Transl. Med. 2017;15;9(377):eaai8700

Horne BD, Muhlestein JB, and Anderson JL. Health effects of intermittent fasting: Hormesisorharm? A systematic review. Am J ClinNutr. 2015; 102:464–470.

Davis CS, Clarke RE, Coulter SN, Rounsefell KN, Walker RE, Rauch CE, Huggins CE, Ryan L. Intermittent energy restriction and weight loss: A systematic review. Eur J Clin. Nutr. 2016;70:292–299.

Zhou Z, Kang YJ. Cellular and Subcellular Localization of Catalasein the Heart of Transgenic Mice. The Journal of Histochemistry & Cytochemistry. 2000; 48(5):585–594.

Ellman GL.Tissue sulphydryl groups. Arch Biochem Biophys. 1959;82:70-77.

Magnani L, Gaydou M, Jean CH. Spectrophotometric measurement of antioxidant properties of flavones and flavonols against superoxide anion. Anal Chim Acta. 2000;411(1-2) 1:209-216.

Giovanni M, Federica F, Roberta S, Cristina B, Sandro A, Roberto FGlucose intolerance and diabetes mellitus in ulcerative colitis: Pathogenetic and therapeutic implications. World J Gastroenterol. 2014;20(13):3507–3515. DOI: 10.3748/wjg.v20.i13.3507.

Hagar H, El-Medany A, El-Eter E, Arafa M. Ameliorative effect of pyrrolidinedithio carbamate on acetic acid-induced colitis in rats. Eur J Pharmacol. 2007;554(1):69-77. DOI: 10.1016/j.ejphar.2006.09.066.

Ige SF and Adio OT. Effect of restraint stress on the reactivation of colitis in acetic acid-induced ulcerative colitis rat. J Adv in Med and Med Res. 2020;32:73-82. Available:https://doi.org/10.934/jammr/2020/v32i930486

Takizawa H,Shintani N, Natsui M, Sasakawa T, Nakakubo H, Nakajima T,Asakura H. Activated immune competentcells in rat colitis mucosa inducedby dextran sulfate sodium and not complete but partial suppression ofcolitis by FK506. Digestion. 1995;56:259–264.

Lars S, Vendahl, Louis E. Underwood, Kaaren M. Haldeman, Martin H. Ulshen, and P. Kay Lund .Fasting Prevents Experimental Murine Colitis Produced by Dextran Sulfate Sodium and Decreases Interleukin-1band Insulin-Like Growth Factor I Messenger Ribonucleic Acid. Endocrinology. 1997;138(2):734-40. DOI: 10.1210/endo.138.2.4941.

Carmen S, LeBlanc A, Martin R, Chain F, Langella P, Bermude-Humaran L, LeBlanc J. Genetically enigineering immunomodulatory Steptococcus thermophiles strains producing antioxidant enzymes exhibit enhanced anti-inflammatory activities. Appl Environ Microbiol. 2014;80(3):869-877.

Yildiz Y, Serter M, Ek R, Ergin K, Cecen S, Demir E, and Yenisey C. Protective effects of caffeic acid phenethyl ester on the intestinal ischemia-reperfusing injury. Dig Dis Sci. 2009 54(4):738-44. DOI: 10.1007/s10620-008-0405-9.

Rebelo E, Luiz-Fereira A, De Faria F, De Almeida A, Dunder R, Manzo L, Souza A. Inhibition of tumor necrotic factor-alpha and cyclooxygenase-2 by Isatin: a molecular mechanism of protection against TNBS-induced colitis in rats. Chemico-biol Interact. 2014;209:48-55.

Toshihiko O, Tekishi O, Teriki H, Funmika I, Eiko K, Shinji F, Takuya I, Kazuhide H, Yuki I, Kawamura and Taeko D. Intermittent fasting prompted recovery from dextran sulfate sodium-induced colitis in mice. J. ClinBiochemNutri. 2017;61:100-107.