Analgesic Effects of Vigna unguiculata subsepecies dekindtiana in Mice

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Adekunle T. Adegbuyi
Moses A. Akanmu
G. Olayiwola
Abayomi O. Sijuade


In the present study, we investigated the antinociceptive effects of the plant Vigna unguiculata spp dekindtiana using chemical and thermal tests in mice. The peripheral and the central analgesic activities of the methanol extract and its fractions were investigated in-vivo in albino mice using acetic acid induced-writhing test and hot plate models respectively. The result of the central analgesic effect showed that the methanol extract (VUME) at 400 mg/kg produced a significant (p<0.05) delay in reaction time in mice on hot plate compared to the control. Various fractions of the extract showed more potency compared to the crude extract. In acetic writhing model, the extract and the fractions demonstrated dose dependent reduction in writhing reaction induced by acetic acid in mice. The reduction was significant when compared to control which was suggestive of the analgesic effect of the plant. It was also seen that the extract and fractions showed an improved analgesic effect compared to diclofenac used as positive control in this model. Yohimbine (alpha adrenergic receptor antagonist) and cyproheptadine (serotonergic receptor antagonist) reversed the antinociceptic effect of the fractions in the hot plate model demonstrating the possibility of adrenergic and serotonergic involvement in eliciting the analgesic effect. Naloxone on the other hand, caused a reversal only in the butanol fraction meaning that this fraction may contain active principles that may mediate their analgesic effect through the opioid mechanism. In the writhing test, yohimbine abolished the analgesic effect of both hexane and butanol fractions. This may therefore, suggest that the analgesic effect of these fractions may be mediated through adrenergic pathway. In conclusion, the plant V. unguiculata subspecies dekindtiana possesses active principles with potential analgesic activity, establishing the folkloric use of the plant in managing pain.

Vigna unguiculata spp dekindtiana, hot plate model, morphine, acetic writhing model, diclofenac

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How to Cite
Adegbuyi, A. T., Akanmu, M. A., Olayiwola, G., & Sijuade, A. O. (2020). Analgesic Effects of Vigna unguiculata subsepecies dekindtiana in Mice. Journal of Advances in Medical and Pharmaceutical Sciences, 22(7), 10-22.
Original Research Article


Rajak AR, Singh R. Contribution of medicinal plants in economic growth. World Journal of Pharmacy and Pharmaceutical Sciences. 2017;6(11):367-372.

Akerele O. Medical plants and primary health care. An agenda for action. Fitoterapia Lix. 1999;355-363.

Shakya KA. Medicinal plants: Future source of new drugs. International Journal of Herbal Medicinal. 2016;4(4):59-64.

Memfin E, Herbert M, et al. Antimicrobial and wound healing activities of Centrosema pubescens (Leguminosae). JPCS. 2011;1:1-6.

Sher H, Aldosari A, Ali A, Boer HJ. Economic benefits of high value medicinal plants to Pakistan communities: An analysis of current practice and potential. Journal of Ethnobiology and Ethnomedicine, 2014; 10(71):1-16.

Wangchuk P, Tobgay T. Contributions of medicinal plants to the Gross National Happiness and Biodiscovery in Bhutan. Journal of Ethnobiology and Ethnomedicine, 2015;11(48):1-12.

Martel MO, Finan HP, Dolman JA, Subramanian S, Edwards RR, Wasan DA. Jamison NR. Self-reports of medication side effects and pain-related activity interference in patients with chronic pain: A longitudinal cohort study. Pain. 2016;156(6):1092–1100.

Baldini A, Von Korff M, Lin EH. A review of potential adverse effects of long-term Opioid therapy: A practitioner’s guide. Prim Care Companion CNS Disord. 2012;14.

Adegbuyi AT, Akanmu MA, Olayiwola G. Neurobehavioural effects of Vigna unguiculata spp dekindtiana (L.) Walp in mice. Journal of Drug and Pharmaceutical Science. 2019;3(4):32-48.

Akinpelu LA, Adegbuyi AT, Agboola SS, Olaonipekun JK, Olawuni IJ, Adegoke AM, Oyemitan IA, Gbola Olayiwola G. Antidepressant activity and mechanism of aqueous extract of Vigna unguiculata ssp. dekindtiana (l.) walp dried aerial part in mice. International Journal of Neuroscience and Behavioral Science. 2017;5(1):7-18.

Lorke D. A new approach to practical acute toxicity testing, Archi. Toxicology. 1983;54: 275-287.

Le Bars D, Gozariu M, Cadden SW. Animal models of nociception. Pharmacological Reviews. 2001;53(4):597-652.

Espejo EF, Mir D. Structure of the rat’s behaviour in the hot plate test. Behavioural brain research. 1993;56(2):171-176.

Ahmed F Selim MST, Das AK, Choudhuri MSK. Anti-inflammatory and antinociceptive activities of Lippiano diflora Linn; Pharmazie, 2004;59:329-333.

Kiliobas K, Stephen O, Ajayi JA. The in-vivo analgesic activity of aqueous and ethanolic extracts of Anacardium occidentale Linn and Cymbopogon citrates DC. Journal of Medicine in the Tropics. 2011;13(2):115-118.

Hossein H, Younesi HM. Antinociceptive and anti-inflammatory effects of Crocus sativus L. stigma and petal extracts in mice. Biology Medical Central Pharmacology. 2002;2.

Kaushik D, Kumar A, Kaushik P, Rana AC. Analgesic and anti-inflammatory activity of Pinus roxburghii Sarg. Advances in Pharmacological Sciences. 2012;1-6.

Musa YM, Haruna AK, Ilyas M, Yaro AH, Ahmadu AA, Usman H. Phytochemical, analgesic and anti-inflammatory effects of the ethylacetate extract of the leaves of Pseudocedrella kotschyii. African Journal Traditional Complimentary Alternative Medicine. 2008;5:92-96.

Hajare SW, Suresh C, Tandan SK, Sarma J, Lal J, Telang AG. Analgesic and antipyretic activities of Dalbergia sissoo leaves. Indian Journal of Pharmacology. 2000;32:357-360.

Maria Elena NG, Jose Arthur Da SE, Souccar C, Antonio JL. Analgesic and anti-inflammatory activities of the aqueous extract of Plantango major L. International Journal of Pharmacognosy. 1997;35:99-104.

Ndebia EJ, Kamgang R, Nkeh-Chungaganye BN. Analgesic and anti-inflammatory properties of aqueous extract from leaves of Solanum torvum (solanaceae). African Journal of Traditional Complimentary Alternative Medicine. 2007;4(2):240–244.

Collier HOJ, Dinneen LC, Johnson Christine A, Schneider C. The abdominal constriction response and its suppression by analgesic drugs in the mouse. British Journal of Pharmacology and Chemotherapy. 1968;32: 295-310.

Abdulmalik IA, Sule MI, Musa AM, Yaro AH, Abdullahi MI, Abdulkadir MF, Yusuf H. Evaluation of analgesic and anti-inflammatory effects of ethanol extract of Ficus iteophylla leaves in rodents. African Journal Traditional Complementary and Alternative Medicine. 2011;8(4):462-466.

Ferdous M, Rouf R, Shilpi JA, Uddin SJ. Anti-nociceptive activity of the ethanolic extract of Ficus racemosa (Lin). Oriental Pharmacology of Experimental Medicine. 2008;8:93-96.

Vyas S, Agrawal RP, Solanki P, Trivedi P. Analgesic and anti-inflammatory activities of Trigonella foenum-graecum (Seed) extract. Acta Pol. Pharm. 2008;65(4):473-476.

Umar AH, Mabrouk M, Danjuma NM, Yaro A. Studies on the analgesic and anti-inflammatory properties of hydro-alcohol extract of Caralluma dalzielii N.E.Br (Asclepiadaceae) in rats and mice. British Journal of Pharmacology and Toxicology; 2013;4(5):169-175.

Sijuade AO. In-vivo Evaluation of analgesic activities of Phyllanthus niruri leaf methanol extract in experimental animal models. Journal of Advances in Medical and Pharmaceutical Sciences. 2016;8(3):1-8.

Sijuade AO, Fadare JO, Oseni OA. Evaluation of anti-inflammatory and

analgesic activities of Tithonia diversifolia in experimental animal models. British Journal of Medicine and Medical Research. 2016; 15(3):1-8

Khan MA, Khan H, Khan S, Mahmood T, Khan PM, Jabar A. Anti-inflammatory, analgesic and antipyretic activities of Physalis minima Linn. Journal of Enzyme Inhibition Medicine. 2009;24(3):632-637.

Buerkle H, Yaksh T. Pharmacological evidence for different alpha2-adrenergic receptor sites mediating analgesia and sedation in the rat. British Journal of Anaesthesia. 1998;81:208–215.

Giovannitti Joseph A, Thoms-Sean M, Crawford James J. Alpha-2 adrenergic receptor agonists: A review of current clinical applications. Anesthesiology programme. 2015;62:31–38.

Morales L, Perez-Garcia C, Alguacil FL. Effects of yohimbine on the antinociceptive and place conditioning effects of opioid agonists in rodents. British Journal of Pharmacology. 2001;133:172-178.

Sabina EP, Chandel S, Rasool MK. Evaluation of analgesic, antipyretic and ulcerogenic effect of Withaferin A. International Journal of Integrative Biology. 2009;6(2):52-56.

Ibironke GF, Ajiboye KI. Studies on the anti-inflammatory and analgesic properties of Chenopodium ambrosioides leaf extract in rats. International Journal of Pharmacology. 2007;3:111-115.

Raffa RB, Friderichs E, Shank RP, Cood EE, Vaught JL, Jacob HI, Selva N. Complimentary and synergistic anti-nociceptive interaction between enantiomers of tramadol. Journal of Pharmacology and Experimental Therapeutics. 1993;267:331-340.

Mohamad AliHijazi, Ahmed El-Mallah, Maha Aboul-Ela, Abdalla Ellakany. Evaluation of analgesic activity of Papaver libanoticum extract in mice: Involvement of Opioids receptors. Evidence-Based Complementary and Alternative Medicine. 2017;1-13: 8935085.

Millan MJ, Newman-Tancredi A, Audinot A, Cussac D, Lejeune F, Nicolas JP, Coge F, Galizzi JP, Boutin JA, Rivet JM, Dekevne A, Gobert A. Agonist and antagonist actions of yohimbine as compared to fluparoxan at alpha(2)-adrenergic receptors (AR)s, serotonin (5-HT)(1A), 5-HT(1B), 5-HT(1D) and dopamine D(2) and D(3) receptors. Significance for the modulation of frontocortical monoaminergic transmission and depressive states. Synape. 2000;35(2): 79-95.

Liu Hai-Bo, Peng Yong, Huang Lu-qi, Xu Jun and Xiao Pei-Gen. Mechanism of selective inhibition of yohimbine and its derivatives in adrenoceptor