Assessment of Atherogenic Indices and Markers of Cardiac Injury in Albino Rats Orally Administered with Tartrazine Azo Dye

Main Article Content

Geraldine Iroh
Benita Oyoburuoma Weli
Uyota Anthony Adele
Ojoye Ngoye Briggs
Helen Anthony Waribo
Ibioku Elekima

Abstract

Aim: Assess the effect of tartrazine azo dye on atherogenic indices and markers of cardiac injury in albino rats.

Study Design: A total number of 63 rats were used for the study. The study was divided into two phases, 1 and 2, which lasted for 30 and 60 days respectively. Phase 1 had 35 rats, 20 as test and 15 as control, while phase 2 had 28 rats, 16 as test and 12 as control. In each phase the test groups were given 7.5mg/kg of tartrazine orally on daily basis over the stipulated period while the control groups were not treated with tartrazine.

Methodology: At the end of the study, 5ml of whole blood was collected from the jugular veins into Lithium Heparin bottles. The sample was spun, plasma collected and analyzed for cardiac Troponin I (cTn-I) and cardiac Troponin T (cTn-T), Total creatinekinase (CK), creatinekinase MM (CK-MM), and creatinekinase MB (CK-MB). Lipid parameters like total cholesterol (TC), High density lipoprotein-cholesterol (HDL-C), low density lipoprotein-cholesterol (LDL-C) and Triglyceride (TG) which were also analysed. Atherogenic indices such as atherogenic coefficient (AC), atherogenic index of plasma (AIP), Non High density lipoprotein-cholesterol (nHDL-C), and castelli risk indices 1 and 2 (CRI-1 and CRI-2) were also calculated. In addition, cardiac tissues were collected, fixed in 10% formol saline and examined histologically using Haematoxylin and Eosin stain. Statistical analysis was performed using GraphPad Prism version 8.02.

Results: The results obtained indicate significant increases in nHDL-C, total CK and cTn-T after 30 and 60 days of treatment with tartrazine at ADI doses against controls. Other atherogenic indices such as AIP, AC, CRI-1 and CRI-2 as well as markers of cardiac injury such as cTn-I and CK-MB indicated non-significant increases.

Conclusion: Orally administered tartrazine over a 60 day period induced cardiac injury as shown by the significant increase in the cTn-T and total CK as well as hypertrophied nuclei of cardiomyocytes. This goes to say chronic administration of tartrazine even at the recommended daily dose could pose the risk of cardiovascular disease. This is also supported by an increase in nHDL cholesterol.

Keywords:
Tartrazine, azo dye, cardiac markers, atherogenic indices, lipid profile, anti-oxidant enzymes, oxidative stress, albino rats.

Article Details

How to Cite
Iroh, G., Weli, B. O., Adele, U. A., Briggs, O. N., Waribo, H. A., & Elekima, I. (2020). Assessment of Atherogenic Indices and Markers of Cardiac Injury in Albino Rats Orally Administered with Tartrazine Azo Dye. Journal of Advances in Medical and Pharmaceutical Sciences, 22(6), 51-61. https://doi.org/10.9734/jamps/2020/v22i630179
Section
Original Research Article

References

Elekima I, Nwachuku EO. Evaluation of acute and chronic toxicity of tartrazine (E102) on steroid reproductive hormones of Albino rats. Asian Journal of Research and Reports in Endocrinology. 2019;2(1):1 -15.

Aberoumand A. A review article on edible pigment properties and sources as national biocolourants in foodstuffs and food industry. World Journal of Dietary and Food Sciences. 2011;6(1):71-78.

Okafor SN, Obonga W, Ezeokonkwo M, Nurudeen J, Orovwigho U, Ahiabuike J. Assessment of the health implications of synthetic and natural food colourants. A critical review. Pharmaceutical and Biosciences. 2016;4(4):01-11.

Elekima I. Effect of carmoisine orally administered on lipid parameters of albino rats. International Journal of Science and Research. 2016;5(9):861-864.

El-Desoky GE, Abdel-Ghaffa A, Al-Othman ZA, Habila MA, Al-Sheikh YA, Ghneim HK, Giesy JP, Aboul-Soud MA. Curcumin protects against tartrazine mediated oxidative stress and hepatoxicity in male rats. European Review for Medical and Pharmaceutical Sciences. 2017;21(3):635-645.

Umbuzeiro GA, Freeman HS, Warren SH, Oliveria DP, Terao V, Watenabe T, Claxton LD. The contribution of azo dyes to the mutagenic activity of the Cristais River. Chemosphere. 2005;60(1): 555–64.

Amin AK, Hameid II AH, Abd-Elsstar HA. Effects of food azo dyes tartrazine and carmosine on biochemical parameter related to renal, hepatic function and oxidative stress biomarkers in young male rats. Food and Chemical Toxicology. 2010;48:2994–3999.

Leo L, Nanyang C, Liu HX, Fai M, Tia MY, Mun W. Occurrence of azo food dyes and their effects on cellular inflammatory Responses. Nutrition. 2018;46:36-40.

Moutinho ILD, Bertges LC, Assis RVS. Prolonged use of the food dye tartrazine (FD& C Yellow No 5) and its effects on the gastric mucosa of wistar rats. Brazillian Journal of Biology. 2007;6(1):141-145.

Chung KT. Mutagenicity and carcinogenicity of aromatic amines metabolically produced from azo dyes. Journal of Environmental Science and Health, Part C. 2008;18(1):51-74.

Elekima I, Nwachuku EO, Ben-Chioma AE. Effect of tartrazine orally administered on thyroid hormones and thyroid stimulating hormone of albino rats. European Journal of Pharmaceutical and Medical Research. 2017;4(7):168-171.

Mehedi N, Mokrane N, Alami O, Ainad-Tabet S, Zaoui C, Kheroua O, Saidi D. A thirteen week ad libitum administration toxicity study of tartrazine in Swiss mice. African Journal of Biotechnology. 2013;12(28):4519–4529.

Himri I, Bellahcen S, Souna F, Belmekki F, Aziz M, Bnouham M, Zoheir J, Berkia Z, Mekhfi H, Saalaoui E. A 90-days oral toxicity of tartrazine; a synthetic food dye, in wistar rats. International Journal of Pharmacy and Pharmaceutical Science. 2010;3(3):159–169.

El-Rabey AH, Al-Seeni NM, Al-Seeni AI, Al-Hamed MA, Zamzami AM, Almutairi MF. Honey attenuates the toxic effect of the low dose of tartrazine in male rats. Journal of Food Biochemistry. 2019;43(4).

Available:https://doi:org/10.1111/jfbc/127802714/19

Accessed 25 June 2020.

Upadhyay RR. Emerging risk biomarkers in cardiovascular disease and disorders. Journal of Lipids. 2015;10:11-15.

Arjmand G, Farzard S, Marzieh MN, Abdullah A. Anthropometric indices and their relationship with coronary artery diseases. Health Scope. 2015;4(3):25-30.

Ukpabi JO, Uwanurochi K. Comparing indications for cardiovascular admissions into a Nigerian and Isreali hospital. Annals of African Medicine. 2017;16(2):70-73.

Luo X, Wu J, Jing S, Yan LJ. Hyperglycemic stress and carbon stress in diabetic glucotoxicity. Aging Diseases. 2016;7:90–110.

Tsikas D. Assessment of lipid peroxidation by measuring malondialdehyde (MDA) and relatives biological samples: Analytical and biological challenges. Analytical Biochemistry. 2017;524:13-30.

Elekima I, Inokon A. A study of correlation of anthropometric data with atherogenic indices of students of rivers state university, Port Harcourt, Nigeria. Asian Journal of Research in Medical and Pharmaceutical Sciences. 2019;6(1):1-12.

Myat SB, Whyte LC, Soe L, Tin MN, Than TW, Myint A. Understanding the relationship between atherogenic index of plasma and cardiovascular disease risk factors among staff of an University in Malaysia. Journal of Nutrition and Metabolism; 2018.

Available:http://doi:org/10.1155/2018/7027 6624

Accessed 29 December 2019

Dobiasova M. Atherogenic index of plasma [log triglyceride/high density lipoprotein cholesterol]: Theoretical practical implications. Clinical Chemistry. 2004;50: 1113-1115.

Devadawson C, Jayasinghe C, Ramiah S, Kanagasingam A. Assessment of lipid profile and atherogenic indices for cardiovascular disease risk based on different fish consumption habits. Asian Journal of Pharmaceutical and Clinical Research. 2016;9(4):156-159.

Brehm A, Pfeiler G, Pacini G, Vierhapper H, Roden M. Relationship between serum lipoprotein ratios and insulin resistance in Obesity. Clinical Chemistry. 2004;50:2316-2322.

Koleva ID, Andreeva-Gateva AP, Orbetzova MM, Atanassovaz BI, Nikolova GJ. Atherogenic index of plasma, castelli risk indexes and leptin/adiponectin ratio in women with metabolic syndrome. International Journal of Pharmaceutical and Medical Research. 2015;3(5):12-16.

Maynard DS, Menown ABI, Adgey AAJ. Troponin T or troponin I as cardiac markers in ischemic heart disease. Heart. 2000;83(4):371.

Available:http://dx.doi.org/10.1136/heart.83.4.371

Accessed 28 Feb. 2020

Dawie J, Chawla R, Worku Y, Azazh A. Diagnosis of Ischemic heart disease using CK-MB, Troponin I and Ischemic modified albumin. Ethiopian Medical Journal. 2011;49(1):25-33.

Engvall E, Perlmann P. Enzyme- linked immunosorbent assay (ELISA) quantitative assay of immunoglobulin G. Immunochemistry. 1971;8(9):871–874.

Flegg HM. An Investigation of the determination of serum cholesterol by an enzymatic method. Annals of Clinical Biochemistry. 1973;10:79-80.

Stavropoulous WS, Crouch RD. A new colourimetric procedure for the determination of serum triglycerides. Clinical Chemistry. 1975;20:857-858.

Friedewald WT, Levy RI, Friedrickson DJ. Estimation of the concentration of low density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clinical Chemistry. 1972; 18(6):499-502.

Elekima I, Ben-Chioma A. Effect of tartrazine orally administered on some atherogenic indices of albino rats. European Journal of Pharmaceutical and Medical Research. 2018;5(11):69-74.

Oyewole IS, Oladele OJ. Assessment of cardiac and renal functions in wistar albino rats administered carmoisine and tartrazine. Advances in Biochemistry. 2016;4(3):21-25.

Amin MAF. Pathophysiological effect of azorubine on female reproductive organs and hormones in Sprague dawley rats. International Journal of Medical Research and Health Science. 2018;7(6):57–62.

Uyota AA, Iroh G, Briggs ON, Waribo AH, Elekima I. Evaluation of anti-oxidant enzymes, lipid peroxidation, lipid profile and liver function in albino rats orally administered tartrazine. International Journal of Biochemistry Research & Review. 2020;29(5):19-29.

Sexena B. Ovarian toxicity and oxidative stress induced by food colours in albino rats. International Journal of Science and Nature. 2016;7(4):838– 842