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Chloroform inhalation is a common method of rodent euthanasia in Nigeria for research purposes. This work is designed to evaluate the consequence of this method of sacrifice on the cerebellum and hippocampus of Wistar rats. Twenty male Wistar rats weighing between 160 and 180 were divided into 4 groups of 5 rats each. Group A served as control and was sacrificed using cervical dislocation (a widely acceptable non-inhalation method of sacrifice). Groups B, C and D were exposed to chloroform for 5 minutes once a day (group B), once a day for 2 days and once a day for 3 days. The brains were removed; four from each group was processed for antioxidant assay while one from each group was fixed in Bouin’s fluid for histological studies. Our results show that chloroform inhalation adversely affected the results of the antioxidant parameters studied in a dose-dependent fashion. That means that the adverse effect worsened as the number of days increased. This was also the case with the histology results as there was evidence of cell necrosis in the cerebellar and hippocampal tissues. This also showed dose dependence. We therefore conclude from our results that when studying the brain tissues or carrying out brain related researches, chloroform inhalation is not the method of choice for rat euthanasia.
Abadin H, Ashizawa A, Stevens YW, Llados F, Diamond G, Sage G, Citra M, Quinones A, Bosch SJ, Swarts SG. Agency for toxic substances and disease registry (ATSDR) toxicological profiles. Toxicological Profile for Lead. Atlanta (GA): Agency for Toxic Substances and Disease Registry (US); 2007.
Bank HS. National library of medicine. Bethesda, Maryland (TOMES CPS# CD-ROM); 1998.
World health Organization (WHO). Environmental Health Criteria 163; Chloroform. International Programme on Chemical Safety. World Health Organization, Geneva; 1994.
US Department of Health and Human Services. Hazardous Substances Data Bank (HSDB, online database). National Toxicology Information Program, National Library of Medicine, Bethesda, MD; 1993.
Smoke T, Smoking I. IARC monographs on the evaluation of carcinogenic risks to humans. IARC, Lyon. 2004;1-452.
Ryder RD. Animal revolution: Changing attitudes towards speciesism. Animal Welfare. 2001;10(2):222.
Duckles SP, Lorraine Sunday, Christa Osuna, Diana N. Krause and Sue P. Duckles. Department of Pharmacology, School of Medicine University of California, Irvine Irvine, CA 92697-4625.
Mizruchi SL. The science of sacrifice: American Literature and Modern Social Theory. Princeton University Press; 1998.
Von Staden H, Chalcedonius H. Herophilus: The art of medicine in early Alexandria: Edition, translation and essays. Cambridge University Press; 1989.
Ryder OA, McLaren A, Brenner S, Zhang YP, Benirschke K. DNA banks for endangered animal species. Science. 2000;288(5464):275-7.
Jarungsriapisit J, Moore LJ, Mæhle S, Skår C, Einen AC, Fiksdal IU, Morton HC, Stefansson SO, Taranger GL, Patel S. Relationship between viral dose and outcome of infection in Atlantic salmon, Salmo salar L., post-smolts bath-challenged with salmonid alphavirus subtype 3. Veterinary Research. 2016;47(1):1-3.
Croce P. Vivisection or science? An investigation into testing drugs and safeguarding health; 1999.
LaFollette H, Shanks N. Animal experimentation: The legacy of Claude Bernard. International Studies in the Philosophy of Science. 1994;8(3):195- 210.
Marquardt N, Feja M, Hünigen H, Plendl J, Menken L, Fink H, Bert B. Euthanasia of laboratory mice: Are isoflurane and sevoflurane real alternatives to carbon dioxide? PLoS One. 2018;13(9):e0203793.
Ekroth G. Animal sacrifice in antiquity. The Oxford Handbook of Animals in Classical thought and Life. 2014;324-54.
American Veterinary Medical Association. AVMA guidelines for the euthanasia of animals: 2013 Edition. American Veterinary Medical Association, Schaumburg, IL; 2013.
Andrew DP, Pedersen PM, McEvoy CD. Research methods and design in sport management. Human Kinetics; 2019.
Keijzer C, de Lange JJ, Perez RS. General introduction: Interactions of inhalational anesthetics and carbon dioxide absorbents. Interactions of Inhalational Anesthetics and Carbon Dioxide Absorbents. 2007;9.
Carbone L, Carbone ET, Yi EM, Bauer DB, Lindstrom KA, Parker JM, Austin JA, Seo Y, Gandhi AD, Wilkerson JD. Assessing cervical dislocation as a humane euthanasia method in mice. Journal of the American Association for Laboratory Animal Science. 2012;51(3):352-6.
Britain G. Guidance on the operation of the animals (Scientific Procedures) Act 1986. Proquest LLC; 2000.
National Research Council, Committee on Acute Exposure Guideline Levels. Acute exposure guideline levels for selected airborne chemicals. National Academies Press. 2009;9.
Duvernoy HM. The human brain stem and cerebellum: Surface, structure, vascularization and three-dimensional sectional anatomy with MRI. Springer Science & Business Media; 2012.
Eichenbaum H. Memory, amnesia and the hippocampal system. MIT Press; 1993.
VanElzakker M, Fevurly RD, Breindel T, Spencer RL. Environmental novelty is associated with a selective increase in Fos expression in the output elements of the hippocampal formation and the perirhinal cortex. Learning & Memory. 2008;15(12): 899-908.
Gornall AG, Bardawill CJ, David MM. Determination of serum proteins by means of the biuret reaction. Journal of Biological Chemistry. 1949;177(2):751-766.
Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. Journal of Biological Chemistry. 1972;247(10):3170-3175.
Ashru K. Sinha. Determination of catalase activity in white blood. Advance in Enzymology and Related Areas of Molecular Biology. 1971;27:380.
Ádám-Vizi V, Seregi A. Receptor independent stimulatory effect of noradrenaline on Na, K-ATPase in rat brain homogenate: Role of lipid peroxidation. Biochemical Pharmacology. 1982;31(13):2231-6.
Fillion M, Mergler D, Passos CJ, Larribe F, Lemire M, Guimarães JR. A preliminary study of mercury exposure and blood pressure in the Brazilian Amazon. Environmental Health. 2006;5(1):29.
Rice KM, Walker Jr. EM, Wu M, Gillette C, Blough ER. Environmental mercury and its toxic effects. Journal of Preventive Medicine and Public Health. 2014;47(2):74.
Monteiro DA, Rantin FT, Kalinin AL. Dietary intake of inorganic mercury: Bioaccumulation and oxidative stress parameters in the neotropical fish Hoplias malabaricus. Ecotoxicology. 2013;22(3): 446-56.
Chen YW, Huang CF, Yang CY, Yen CC, Tsai KS, Liu SH. Inorganic mercury causes pancreatic β-cell death via the oxidative stress-induced apoptotic and necrotic pathways. Toxicology and Applied Pharmacology. 2010;243(3):323-31.
Graeme KA, Pollack Jr. CV. Heavy metal toxicity, Part I: Arsenic and mercury. The Journal of Emergency Medicine. 1998;16(1):45-56.
Gilboa A, Winocur G, Rosenbaum RS, Poreh A, Gao F, Black SE, Westmacott R, Moscovitch M. Hippocampal contributions to recollection in retrograde and anterograde amnesia. Hippocampus. 2006;16(11):966-80.