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Aim: The study is targeted at the sickle cell disease. It has been discovered that some drugs or medications taken for certain ailments are either pro-sickling or anti-sickling in nature. In this study, acorticosteroid by the name of prednisolone was investigated to determineits possible effects on human haemoglobin-S gelation, erythrocyte fragility and Fe2+ and Fe3+ concentrations.
Materials and Method: The blood sample of 5ml was collected from adult male and female donors by vein puncture using a 5 ml syringe and needle. The blood samples were confirmed as HbSS using standard haemoglobin electrophoresis. Various concentrations of the drug (0.05, 0.1, 0.3, 0.5 and 1 mg/ml) were used to determine the effects on human haemoglobin-S, gelation rate, erythrocyte fragility, Fe2+& Fe3+ concentrations. Absorbance reading was taken at 540 nm using a spectrophotometer.
Results: The results showed that Prednisolone increased haemoglobin S gelation at all concentrations (p< 0.05) when compared to the control. The Fe2+/Fe3+ ratio showed a reduction in haemoglobin values at 0.3, 0.5 and 1.0 mg/ml concentrations when compared to the control and a slight increase at 0.05 and 0.1 mg/ml. For Erythrocyte Fragility, there was destabilization of red cell in all concentrations.
Conclusion: This study suggests that this drug could have some undesirable effects on sickle cell subjects.
Yazdan B, Lomas K, Francis C, Reid ME. Blood groups and diseases associated with inherited abnormalities of the red blood cell membrane transfusion medicine. Reviews. 2000;14(4):364-47.
Adams RJ. TCD in sickle cell disease: An important and useful test paediatric radiolo. 2005;35:229-34.
Akinyanju O, Akinsete I. Legulceration in sickle cell disease in Nigeria. Trop and Geo Med. 1979;31:87-91.
Almeida A, Roberts I. bone involvement in sickle cell disease. Brit J of Haem. 2005; 129:482-90.
Ulker P, Siti L, Ceilik O, Ozenic C, Meiselma HJ, Baskurt OK. Mechanical stimulation of erythrocytes. Biorheral. 2009;46(2):121-32.
Lee SH. Mechanisms of glucocorticoid action in chroniz rhinosinusilis. Aller Asthma & Immuno Res. 2015;7(6):534-7.
Huck VM. Sickle cell anaemia. Bull John’s Hopk Hosp. 1993;34:334(1923).
Ataga KI, Orringer EP. Renal abnormalities in sickle cell disease. Am J of Hema. 2000; 63:205-11.
Biagoli M, Pinto M, Cesselli D. Unexpected expression of alpha-and beta-globin in messencephatic dopaminergic neurons and giat cells. Prol Natl Acad Scie. U.S.A. 2009;106(36):15454-9.
Free SB, Vincken W. Systemic corticosteroid therapyfor acute asthma exacerbations. J. Asthma. 2006;43(5): 321-31.
Ballas SK, Lusardi M. Pain management of sickle cell disease. Haemclin of North Am. 2005;19:785-802.
Anie KA. Physiological complications in sickle cell diseases. Brit J of Haem. 2005; 129:723-9.
Kabanova S, Kleinbongard P, Volkmer J, Andree B, Kelm M, Jax TW. Gene expression analysis of human red blood cells. Inter J of Med Sci. 2009;6(4):156-9.
Uwakwe AA, Onyeike EN. Effect of nicotimic acid on haemoglobin-s (Hbs) gelatwn and osmotic fragility of Abs Erjthrocytes. Glo J. Med. Sci. 2005;2(1): 59-63.
Stahn C. Molecular mechanisms of glucocorticoid action and selective glucocorticoid receptor agonists (PDF). Mol and cell endocrinol. 2007;275:71- 78.
Daland GA, Castle WB. A simple and rapid method for demonstrating sickling of the red blood cells. The use of reducing agents. J. Lab. Clin. Med. 2007;33:1085.
Dacie JV, Lewis SM, Luzzatto L. Investigation of the hereditary haemolyti-canaemia membrane and enzyme abnormalities in practical haemology. Churchill Livingstone New York. 1981;234–238
Noguchi CT, Schechter AN. Sickle haemoglobin polymerization in solutions and in cells. Ann Rev of Biophys Chem. 1985;14:239–246.
Chikezie PC, Akuwudike AR, Chikezie CM. Polymerisation studies of sickle cell haemoglobin incubated in aqueous leaf extract of Nicotianatabacum product. Res J of Med Plant. 2013;7:92–99.
Ibegbulem CO, Eyong EU, Essien EU. Polymerisation inhibition activity of Raphiahooken palm sap and its effect on osmotic fragility of sickle cell red blood cells. J of Med Plant Res. 2011;5:4212–4217.
Miera M, Tamame T, Naganuma T, Chinen S, Matsuoka M, Ohki H. Steriod pulse therapy for kawasaki disease unresponsive to additional immunoglobulin therapy. Paed and Child Hea. 2011;16(8):479-84.
Uwakwe AA, Akhidue V. The effect of quinine on human haemoglobin – S (HbS) erythrocyte sickling and HbS gelation rate. J ApplSci Environ Mgt. 2000;2(4):75–77.
Uwakwe AA, Onwuegbuke C, Nwinuka NM. Effect of caffeine on the poly-merisation of HbS erythrocytes. J Appl Sci Environ Mgt. 2002;6(1):69–72.
Okoye NF, Uwakwe AA, Ayalogu EO. Investigation of the in vitro effects of some oral contraceptives on human HbSS erythrocyte fragility and gelation. The Inter J of Sci and Tech. 2017;5(7):27-31.
Chikezie PC, Uwakwe AA. Membrane stability of sickle erythrocyte incubated in incubated in the extract of three medicinal plants. Anacardium occidentale, Psidium guajava and Terminalia catappa. Pharm. Mag. 2011;7:121–125.
Ekeke OI, Uwakwe AA, Nwaoguikpe. Edible Legumes as Nutritionally Beneficial Antisickling Agents Nig. J. Biochem Mol. Bio. 2000;15:111-113.
Chikezie PC. Sodium metabisulfite induced polymerisation of sickle cell haemoglobin incubated in the extract of three medicinal plants. Anacardium occidentale, Psidium guajava and Terminalia catappa. Pharm. Mag. 2011;7:126–132.
Bianchi N, Zucatto C, Lampronti I, Borgatti M, Gambari R. Fetal haemoglobin inducers from natural world. A novel approach for identification of drugs for the treatment of β thalassemia and sickle cell amaemia. Evid Based Com of Alter Med. 2009;6:141– 151.
Lambrou GI, Viahopoulos S, Papathanasiou C, Papanikoloau M. Pred-nisolone exerts late mitogenic and biphasic effects on resistant acute lymphoblastic leukemia cells: Relation to early gene expression. Leuk Res. 2009;33(12):1684-95.