In vitro-Modulation of HbS Erythrocyte Parameters By Prednisolone Testing For Fe2+/Fe3+ Ratio, HbS Gelation and Osmotic Fragility

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Okoye Ngozi Franca
Amadi Benjamin Achor
Okoro Miriam


Aim: The study is targeted at the sickle cell disease. It has been discovered that some drugs or medications taken for certain ailments are either pro-sickling or anti-sickling in nature. In this study, acorticosteroid by the name of prednisolone was investigated to determineits possible effects on human haemoglobin-S gelation, erythrocyte fragility and Fe2+ and Fe3+ concentrations.

Materials and Method: The blood sample of 5ml was collected from adult male and female donors by vein puncture using a 5 ml syringe and needle. The blood samples were confirmed as HbSS using standard haemoglobin electrophoresis. Various concentrations of the drug (0.05, 0.1, 0.3, 0.5 and 1 mg/ml) were used to determine the effects on human haemoglobin-S, gelation rate, erythrocyte fragility, Fe2+& Fe3+ concentrations.  Absorbance reading was taken at 540 nm using a spectrophotometer.

Results: The results showed that Prednisolone increased haemoglobin S gelation at all concentrations (p< 0.05) when compared to the control. The Fe2+/Fe3+ ratio showed a reduction in haemoglobin values at 0.3, 0.5 and 1.0 mg/ml concentrations when compared to the control and a slight increase at 0.05 and 0.1 mg/ml. For Erythrocyte Fragility, there was destabilization of red cell in all concentrations.

Conclusion: This study suggests that this drug could have some undesirable effects on sickle cell subjects.

Erythrocyte, fragility, gelation, haemoglobin, prednisolone, sickle cell.

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How to Cite
Franca, O., Achor, A., & Miriam, O. (2019). In vitro-Modulation of HbS Erythrocyte Parameters By Prednisolone Testing For Fe2+/Fe3+ Ratio, HbS Gelation and Osmotic Fragility. Journal of Advances in Medical and Pharmaceutical Sciences, 20(4), 1-7.
Original Research Article


Husebye ES, Allolio B,Arit W, Badenhoop K, Bensing S, Betterle C, Falovni A, Gan EH, Hutting AL. Consensus statement on the diagnosis, treatment and follow-up of patients with primary adrenal insufficiency. J of Internal Med. 2014;275(2):104-115.

Yazdan B, Lomas K, Francis C, Reid ME. Blood groups and diseases associated with inherited abnormalities of the red blood cell membrane transfusion medicine. Reviews. 2000;14(4):364-47.

Adams RJ. TCD in sickle cell disease: An important and useful test paediatric radiolo. 2005;35:229-34.

Akinyanju O, Akinsete I. Legulceration in sickle cell disease in Nigeria. Trop and Geo Med. 1979;31:87-91.

Almeida A, Roberts I. bone involvement in sickle cell disease. Brit J of Haem. 2005; 129:482-90.

Ulker P, Siti L, Ceilik O, Ozenic C, Meiselma HJ, Baskurt OK. Mechanical stimulation of erythrocytes. Biorheral. 2009;46(2):121-32.

Lee SH. Mechanisms of glucocorticoid action in chroniz rhinosinusilis. Aller Asthma & Immuno Res. 2015;7(6):534-7.

Huck VM. Sickle cell anaemia. Bull John’s Hopk Hosp. 1993;34:334(1923).

Ataga KI, Orringer EP. Renal abnormalities in sickle cell disease. Am J of Hema. 2000; 63:205-11.

Biagoli M, Pinto M, Cesselli D. Unexpected expression of alpha-and beta-globin in messencephatic dopaminergic neurons and giat cells. Prol Natl Acad Scie. U.S.A. 2009;106(36):15454-9.

Free SB, Vincken W. Systemic corticosteroid therapyfor acute asthma exacerbations. J. Asthma. 2006;43(5): 321-31.

Ballas SK, Lusardi M. Pain management of sickle cell disease. Haemclin of North Am. 2005;19:785-802.

Anie KA. Physiological complications in sickle cell diseases. Brit J of Haem. 2005; 129:723-9.

Kabanova S, Kleinbongard P, Volkmer J, Andree B, Kelm M, Jax TW. Gene expression analysis of human red blood cells. Inter J of Med Sci. 2009;6(4):156-9.

Uwakwe AA, Onyeike EN. Effect of nicotimic acid on haemoglobin-s (Hbs) gelatwn and osmotic fragility of Abs Erjthrocytes. Glo J. Med. Sci. 2005;2(1): 59-63.

Stahn C. Molecular mechanisms of glucocorticoid action and selective glucocorticoid receptor agonists (PDF). Mol and cell endocrinol. 2007;275:71- 78.

Daland GA, Castle WB. A simple and rapid method for demonstrating sickling of the red blood cells. The use of reducing agents. J. Lab. Clin. Med. 2007;33:1085.

Dacie JV, Lewis SM, Luzzatto L. Investigation of the hereditary haemolyti-canaemia membrane and enzyme abnormalities in practical haemology. Churchill Livingstone New York. 1981;234–238

Noguchi CT, Schechter AN. Sickle haemoglobin polymerization in solutions and in cells. Ann Rev of Biophys Chem. 1985;14:239–246.

Chikezie PC, Akuwudike AR, Chikezie CM. Polymerisation studies of sickle cell haemoglobin incubated in aqueous leaf extract of Nicotianatabacum product. Res J of Med Plant. 2013;7:92–99.

Ibegbulem CO, Eyong EU, Essien EU. Polymerisation inhibition activity of Raphiahooken palm sap and its effect on osmotic fragility of sickle cell red blood cells. J of Med Plant Res. 2011;5:4212–4217.

Miera M, Tamame T, Naganuma T, Chinen S, Matsuoka M, Ohki H. Steriod pulse therapy for kawasaki disease unresponsive to additional immunoglobulin therapy. Paed and Child Hea. 2011;16(8):479-84.

Uwakwe AA, Akhidue V. The effect of quinine on human haemoglobin – S (HbS) erythrocyte sickling and HbS gelation rate. J ApplSci Environ Mgt. 2000;2(4):75–77.

Uwakwe AA, Onwuegbuke C, Nwinuka NM. Effect of caffeine on the poly-merisation of HbS erythrocytes. J Appl Sci Environ Mgt. 2002;6(1):69–72.

Okoye NF, Uwakwe AA, Ayalogu EO. Investigation of the in vitro effects of some oral contraceptives on human HbSS erythrocyte fragility and gelation. The Inter J of Sci and Tech. 2017;5(7):27-31.

Chikezie PC, Uwakwe AA. Membrane stability of sickle erythrocyte incubated in incubated in the extract of three medicinal plants. Anacardium occidentale, Psidium guajava and Terminalia catappa. Pharm. Mag. 2011;7:121–125.

Ekeke OI, Uwakwe AA, Nwaoguikpe. Edible Legumes as Nutritionally Beneficial Antisickling Agents Nig. J. Biochem Mol. Bio. 2000;15:111-113.

Chikezie PC. Sodium metabisulfite induced polymerisation of sickle cell haemoglobin incubated in the extract of three medicinal plants. Anacardium occidentale, Psidium guajava and Terminalia catappa. Pharm. Mag. 2011;7:126–132.

Bianchi N, Zucatto C, Lampronti I, Borgatti M, Gambari R. Fetal haemoglobin inducers from natural world. A novel approach for identification of drugs for the treatment of β thalassemia and sickle cell amaemia. Evid Based Com of Alter Med. 2009;6:141– 151.

Lambrou GI, Viahopoulos S, Papathanasiou C, Papanikoloau M. Pred-nisolone exerts late mitogenic and biphasic effects on resistant acute lymphoblastic leukemia cells: Relation to early gene expression. Leuk Res. 2009;33(12):1684-95.