Tissue Protein Carbonylation in Aging: A Strategic Analysis of Age-Related Protein Modification

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T. A. Kolawole
O. N. Ilochi
B. O. Oluwatayo
A. N. Chuemere
D. V. Dapper


Free radicals generated in a variety of biological systems have been implicated in mechanisms of aging and age-related pathologies. This study strategically revealed the varying levels of carbonylated proteins in 3 different tissues of 40 wistar rats of varying ages. Their ages include 25-30, 45-50 and 65-70 days. The brain, heart and kidney tissue homogenates were prepared and biochemically analyzed for products of protein oxidation using 2,4-dinitrophenylhydrazones and autoantibodies against carbonylated proteins. This study revealed a direct proportional relationship between age and protein carbonylation in brain, heart and kidney tissue homogenates. The level of carbonylated proteins were significantly (P≤0.05) increased in the assayed tissues as all test groups advanced in age. Oxidative modification of proteins in brain and kidney tissues showed similar trend. This age-related biochemical manifestation may be as a result of increased generation of free radicals at mitochondrial level or decreased anti-oxidant defenses as living organisms advance in age.

Aging, carbonylated proteins, free radicals, 2,4-dinitrophenylhydrazone

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How to Cite
Kolawole, T. A., Ilochi, O. N., Oluwatayo, B. O., Chuemere, A. N., & Dapper, D. V. (2019). Tissue Protein Carbonylation in Aging: A Strategic Analysis of Age-Related Protein Modification. Journal of Advances in Medical and Pharmaceutical Sciences, 20(3), 1-5. https://doi.org/10.9734/jamps/2019/v20i330111
Original Research Article


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