The Frequency of CYP3A5 Expression in a Mexican Population Compared to a Caucasian Population
Jason W. Guy
University of Findlay College of Pharmacy, Findlay, Ohio, USA
Arthur F. Harralson
The Bernard J. Dunn School of Pharmacy, Shenandoah University, Winchester, Virginia, USA
Kylie Pooler
The Bernard J. Dunn School of Pharmacy, Shenandoah University, Winchester, Virginia, USA
Robert S. Kidd *
The Bernard J. Dunn School of Pharmacy, Shenandoah University, Winchester, Virginia, USA
*Author to whom correspondence should be addressed.
Abstract
Aims: To determine the frequencies of the six most common CYP3A5 alleles and genotypes in a Mexican-American population compared to a non-Hispanic Caucasian population.
Study Design: This cross-sectional study compared the frequency of CYP3A5 genetic variations in a Mexican-American population compared to a non-Hispanic Caucasian population.
Duration of Study: The study was conducted from September 2014 to April 2015.
Methodology: There were 236 Mexican-American and 237 non-Hispanic Caucasian samples that were analyzed. The two groups of subjects’ gDNA were analyzed for CYP3A5 rs776746 (*3), rs56411402 (*4), rs10264272 (*6), rs41303343 (*7), rs55817950 (*8), and rs28383479 (*9).
Results: There was a significant difference in the CYP3A5*3 containing diplotypes, but no other diplotypes were significantly different. The frequency of the CYP3A5*3 allele in the Mexican population was 0.782, which was significantly lower than the frequency of the CYP3A5*3 allele in the non-Hispanic Caucasian population of 0.932 (P <0.001). The CYP3A5*7 frequency was very low in the Mexican-American group at 0.85% and was absent in the Caucasian group (P = 0.045). Mexican-Americans were three times more likely to be CYP3A5 expressers compared to non-Hispanic Caucasians (P <0.001).
Conclusion: CYP3A5*3 and *7 allele frequencies vary significantly between Mexicans and non-Hispanic Caucasians, while other allele frequencies for CYP3A5*4, *6, *8 and *9 do not vary significantly between Mexicans and non-Hispanic Caucasians. Mexican-Americans were shown to have a three-fold higher frequency of CYP3A5 expression compared to non-Hispanic Caucasians.
Keywords: CYP3A5, Mexicans, pharmacokinetics, drug dosing, allele frequency, genotype