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This research work was focus on determining the quantitative phytochemical analysis and antifungal susceptibility of Azardirachta indica against some strains of Candida albicans. The leaves of Azardirachta indica was susccessively extracted using reflux method of extraction. Quantitative phytochemical screenings were done to determine the amounts of phytochemical that are present in the crude extracts. The result showed that the extract of Azardirachta indica (neem) possessed active principles which include: Alkaloids, saponins, flavonoids, phenols and tannin which were present in the crude extracts. The antifungal activity of the extracts was assayed against three different strains (P37005, RM1000 & SC5314) The antifungal susceptibility test of the crude extracts against the strains were determined at different concentrations of 40, 60, 80 and 100 mg/ml using the agar well diffusion method. The n- hexane and methanol crude extract showed inhibitory activity compared to that of ethyl acetate that had no inhibitory activity. The highest mean zone of inhibition (MZI) was 15.00±1.00 mm which was recorded for n-hexane leaf extract at a concentration of 100 mg/ml against strain RM1000 (isolate: S2). Although the standard antibiotics (Fluconazole) used in this study showed a much higher zone of inhibition than that of the n-hexane and methanol crude extract. The MIC and MFC value for n-hexane crude extracts were 12.5 mg/ml and 50 mg/ml respectively against strain P37005 (Isolate:S1)likewise, the n-hexane crude extract had a value of 6.25 mg/ml and 50 mg/ml. However, the methanol crude extract showed a value of 6.25 mg/ml and 100 mg/ml respectively against SC5314 (isolate:S3).
Biswas K, Chattopadhyay I, Banerjee RK. Biological activities and medical property of neem (Azardirachta indica) U. Current Science. 2002;82:1336-1345.
Chattopadhyay RR, Chattopadhyay RN, Maitra SK. Possible mechanism of anti -inflammatory activity of Azardirachta indica leaf extract. Indian Journal of pharmaco-logy. 1994;26:141-143.
Nat VD, Klerx J, Dijk HV, De Silva KT, Labadie RP. Immunomodulatory activities of an acqueous extract of Azardirachata indica stem bark. Journal of Ethno Pharmacology. 1987;27: 143-145
Sengupta P, Chowdhuri SN, Khastagir HN. Terpenoids and related compounds-1 constituents of the trunk bark of Melia azardirachta Linn and the structure of the ketophenol, nimbol. Tetra Hedron. 1960;10-45-45.
Shumutterer H. The neem tree Azadirachta indica A. Juss and other meliaceous plants. 2nd edition. International print-o-pac limited. Munbai. 2002;893.
Fujiwara T, Sugishita E, Takeda T, Ogihara Y, Shimizu M, Nomura T, Devakumar C. In Neem (eds Randhawa and Parmar, B. S.) 2nd edition. 1996; 77110.
Subramanian MS, Lakshmanan KK. Azadirachta indica A. Juss. Stem bark as antileprosy source. Editors neem and environment. New Delhi: Oxford & IBH Publishing Co Pvt Ltd. 1996;2:1143-1150.
Bokhari MH, Aslam KM. Neem (Melia azadirachta A. Juss). A useful tree in Northern Nigeria. Annals of Bornoll. 1985; 83–86.
Akol AM, Sithanantham S, Varela AM, Mueke JM, Okelo RO. Evaluation of two neem insecticides for nontarget effects on the larvalparasitoids of the diamond back moth, Plutella xylostella; 2001.
Makeri K, Maikai VA, Nok JA. Effect of topical application of neem seed of Azadirachta indica extract on sheep infested with Amblyomma variegatum. African Journal of Biotechnology. 2007; 116:387-401.
John Thorne C, Heidi L, Lawrence CP. Manual of medical mycology. Cambridge, Mass, USA: Blackwell Scientific, C. 1995; 2010.
Cheesbrough M. Laboratory manual. In: District Laboratory Practice in Tropical Countries. UR united Kingdom: Cambridge University Press. 2010;146-157.
National Centre for Biotechnology and Information (NCBI). Sequence BLAST; 1988.
Hymete ATH, Iversen J, Rohloff B, Erko E. Screening of Echinops ellenbeckii and Echinops longisetus for biological activities and chemical constituents. Phytomedicine. 2004;01-03.
Chang C, Yang M, Wen H, Chern J. Estimation of total flavonoid contents in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis. 2002;10:178-182.
Singleton VL, Orthofer R, Lamuela- Raventos RM. Analysis of total phenols and other oxidants substrates and antioxidants by means of Folin -Ciocalteu reagent. Method in Enzymology. 1999;299: 152-178.
Oloyed OI. Chemical profile of unripe pulp of Carica pagaya. Pakistan Journal of Nutrition; 2005.
Emmanuel EO, Helmina O, Egwin CE. Phytochemical constituents of seeds of ripe and unripe Blighia sapida (K Koening) and physicochemical properties of the seed Oil. International Journal of pharmaceutical Science invention. 2014; 3(9):31-40.
Wheeler EL, Ferrel RE. Western regional research laboratory, Agricultural Research Servive, U.S. Department of Agriculture, Albany, California 94710; 1971.
Oke OL. Chemical studies on some Nigeria Food stuffs. Journal of West African Science Association. 1996;11:42-48.
McFarland J. Nephelometer. Journal of the American Medical Association. 1997;14: 1176-1178.
Magaldi S, Mata S, Hartung C. In vitro susceptibility of 137 Candida sp. isolates from HIV positive patients to several antifungal drugs. Mycopathologia. 2002; 149:63–68.
Ewansiha JU, Garba SA, Musa G, Daniyan SY, Busari Innalegwu DA, Doughari JH. Preliminary phytochemical and Anti-microbial Activity of Citrus x limon (L) Burm. f. (lemon) leaf extract against some pathogenic microorganisms. Journal of Applied life Science International. 2016; 6(4):1-10.
Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. African Journal of Biotechnology. 2005;4:685-688.
Oke F, Aslim B. Bological potencials and cytotoxicity of various extracts from endemic Origanum minutiflorum Schwartz O, Davis PH. Food and Chemical Toxicology. 2010;48:1728-1733.
Costa AF. Pharmacognosy: Experimental pharmacognosy. 3rd edition. Lisboa: Calouste Gulbenkian. 2001;3(303):308-309.
Al-Humaid AI, Mousa RA, El-mergawi, Abdel-Salam AM. Chemical composition and antioxidant activity of dates and dates- Camel-Milk mixtures as a protective meal against lipid peroxidation in rats. American Journal of Food Technology. 2010;5:22-30.
Jones GA, McAllister TA, Muir AD, Cheng KJ. Effects of Safonin (Onorbrychis viciifolia scop) condensed tannins on growth and proteolysis by four strains of ruminal bacteria. Appl. Environ Microbiol. 1994;60:1374-1378.
Silver EM, Souza JNS, Rogez H, Rees JF, Larondelle Y. Antioxidant activities and polyphenolic contents of fifteen selected plant species from the Amazonian region. Food Chemistry. 2007;101:1012-18.
Mamta S, Jyoti S, Rajeev N, Dharmendra S, Abhishek G. Phytochemistry of medicinal plant. Journal of Pharmacognosy and Phytochemistry. 2013;1(6):168-180.
Jisika M, Ohigashi H, Nogaka H, Tada T, Hirota M. Bitter steroid glycosides, Vernon sides A1, A2 and A3 and related B1 from the possible medicinal plant Vernonia amygdalina used by Wild Chimpanzees Tetrahedron. 1992;48:625-630.
Hurrel RH, Reddy MB, Juillerat MA, Cook JD. Degredation of phytic acid in cereal porridges improve iron absorption by human subjects. Journal of clinical Nutrition. 2003;77(5):1213-9.
Prescott LM, Harley JP, Klein DA. Microbiology (International edition). 5th edition. New York: McGraw-Hill companies, incorporation. 2002;811.
Mahmoud DA, Hassanein NM, Youssef KA, Abouzeid MA. Antifungal activity of neem leaf extracts and the nimonol against some important human pathogen. Brazilian Journal of Microbiology. 2011;42(3):1007-1016.
Simhadri VS, Nagesh NA, Muniappan M, Kannan I, Viswanathan S, Jayachandra K. Antifungal activity of various extracts of Azardirachta indica leaf – An in vitro study. International Journal of Chem Tech Research. 2007;10(15):305-311.
Wallack JB. Interpretation of diagnostic test. Philadelphia: Lippincott Williams|& Wilkins; 2007.
Calixto JB. Efficacy, safety, quality control, marketing and regulatory guidelines for herbal medicines. Brazilian Journal of Medical and Biological Research. 2002; 33:179-189.
Mann A, Yahaha YA, Banso A, John F. Phytochemical and antimicrobial activities of Terminalia avicenniodes extracts against some bacteria pathogens associated with patients suffering from complicated respiratory tract diseases. Journal of Medicinal plants Research. 2008;2(5):094-097.